Significance of expression of MIF-mRNA in breast cancer

doi:10.3877/cma.j.issn.1674-0807.2008.02.007

Abstract

Abstract:

Objective

To investigate the expression of macrophage migration inhibition factor(MIF) gene in breast cancer by evaluating the expression levels of MIF-mRNA in breast cancer and normal breast tissues.

Methods

Total RNA was extracted from breast cancer tissue and beside-cancer tissue.The MIF- mRNA expressions of breast cancer tissue and beside-cancer tissue of 38 patients were tested by RTPCR,and the relation between them was analyzed.

Results

The MIF- mRNA level in the breast cancer tissue (0.82 ±0.07)was significantly higher than that of beside-cancer tissue (0.57 ±0.03),(P ＜0.05),and significantly higher in patients with positive metastasis of axillary lymph nodes than in patients with negative metastasis of axillary lymph nodes.

Conclusion

MIF,as a cytokine,whose expression increases significantly in breast cancer,might be involved in pathogenesis and metastasis of breast cancer.

Key words: Breast carcinoma, Macrophage migration inhibition factor gene

Shi-jiang WANG, Zhi-jun HUO, Peiguo WANG, Ping WANG. Significance of expression of MIF-mRNA in breast cancer[J]. Chinese Journal of Breast Disease(Electronic Edition), 2008, 02(02): 25-27.

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URL: https://zhrxbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0807.2008.02.007

https://zhrxbzz.cma-cmc.com.cn/EN/Y2008/V02/I02/25

Figures/Tables 3

References 10

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组织	n	MIF/GAPDH
乳腺癌^a	38	0.82±0.07(32例阳性)
癌旁组织	38	0.57±0.03(26例阳性)

组织	n	MIF/GAPDH
乳腺癌^a	38	0.82±0.07(32例阳性)
癌旁组织	38	0.57±0.03(26例阳性)

临床病理因素	n	MIF/GAPDH		检验值
临床病理因素	n	阳性例数	MIF阳性(32例)	检验值
临床分期
Ⅰ	3	3	0.78±0.05
Ⅱ	26	22	0.83±0.07	F=0.78
Ⅲ	9	7	0.80±0.06	P=0.468
淋巴结转移
(-)	18	12	0.78±0.04	P＜0.05
(+)	20	20	0.84±0.08	P＜0.05
月经
未绝经	20	19	0.83±0.07	P=0.841
绝经	18	13	0.81±0.04	P=0.841
ER
(-)	17	14	0.81±0.03	P=0.776
(+～+++)	21	18	0.83±0.07	P=0.776

临床病理因素	n	MIF/GAPDH		检验值
临床病理因素	n	阳性例数	MIF阳性(32例)	检验值
临床分期
Ⅰ	3	3	0.78±0.05
Ⅱ	26	22	0.83±0.07	F=0.78
Ⅲ	9	7	0.80±0.06	P=0.468
淋巴结转移
(-)	18	12	0.78±0.04	P＜0.05
(+)	20	20	0.84±0.08	P＜0.05
月经
未绝经	20	19	0.83±0.07	P=0.841
绝经	18	13	0.81±0.04	P=0.841
ER
(-)	17	14	0.81±0.03	P=0.776
(+～+++)	21	18	0.83±0.07	P=0.776

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