Expressions of matrix metalloproteinase-2 and sialyl Lewis-X in breast carcinoma and the significance

doi:10.3877/cma.j.issn.1674-0807.2008.02.008

Abstract

Abstract:

Objective

To investigate the expressions of matrix metalloproteinase-2 (MMP-2) and sialyl Lewis-X(SLeX) in breast carcinoma and their relationships with lymph node metastasis and prognosis.

Methods

The expressions of MMP-2 and SLeX in breast carcinoma (n =120 cases),metastatic lymph nodes (n =71 cases) and normal epithelial tissue remote from carcinoma (n =30 cases) were studied with microwave-labelled streptavidin biotin immunohistochemical method.

Results

The positive rates of MMP-2 and SLeX expressions were 16.7% (5/30) and 20.0% (6/30)in normal breast tissue,and 78.3%(94/120) and 86.7% (104/120) in breast carcinoma,respectively. The expressions of MMP-2 and SLeX in breast carcinoma were significantly higher than in the normal breast tissues (P ＜0.05). There was no difference in MMP-2 expression in the primary of breast carcinoma between patients with lymphaden metastasis and patients without lymphaden metastasis(P ＞0.05). The positive rate of SLeX in the primary breast carcinoma with lymphaden metastasis was higher than that without lymphaden metastasis(P ＜0.05). The positive rates of MMP-2 and SLeX expressions were 21. 1% (15/71) and 88. 7%(63/71) in metastatic lymph nodes, respectively. The expression of SLeX in metastatic lymph nodes was corresponding to that in the primary of breast carcinoma (r =0.874,P ＜0.05).

Conclusion

MMP-2 and SLeX were both highly expressed in breast carcinoma. SLeX was closely related to lymphaden metastasis. MMP-2 and SLeX,as a tumor-associated antigen in breast carcinoma, can be used as important markers to predict invasion, metastasis and prognosis.

Key words: Breast carcinoma, Matrix metalloproteinase-2, Sialyl Lewis-X, Infiltration; Metastasis

Peizhong SHANG, ZHANG Jin-jiang ,, Guo-hong JIA, Yong-qing LI, Jian-jun MIAO, Xiao-wu LI, Hua-ping GU. Expressions of matrix metalloproteinase-2 and sialyl Lewis-X in breast carcinoma and the significance[J]. Chinese Journal of Breast Disease(Electronic Edition), 2008, 02(02): 28-31.

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References 11

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分组	例数	MMP-2		SLeX
分组	例数	阳性例数	百分率(%)	阳性例数	百分率(%)
正常乳腺组织	30	5	16.7	6	20.0
乳腺癌组织	120	94	78.3	104	86.7
无淋巴转移	49	34	69.4	33	67.3
有淋巴转移	71	60	84.5^a	71	100.0^b

分组	例数	MMP-2		SLeX
分组	例数	阳性例数	百分率(%)	阳性例数	百分率(%)
正常乳腺组织	30	5	16.7	6	20.0
乳腺癌组织	120	94	78.3	104	86.7
无淋巴转移	49	34	69.4	33	67.3
有淋巴转移	71	60	84.5^a	71	100.0^b

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