Abstract:
Objective
To explore the role of p21waf1 gene transfection in the proliferation of human breast carcinoma cells.
Methods
p21waf1 gene packaged with liposome was transfected into the cells of a human breast carcinoma cell line MCF-7,which stably expressing p21waf1gene. The cell growth was detected by MTT. The cell cycle pattern was assayed by flowcytometry.
Results
After transfection of pIRES-p21waf1,p21waf1 mRNA and the protein of MCF-7-p21waf1 were highly expressed compared with control. The cell growth was obviously inhibited and resulted in an accumulation of cells in G1,presenting that the proportion of the cells in G phase was obviously increased from 49.84 ±1.58% to 70.52 ±3.21%, and that of the cells in S and G2/M phases was obviously decreased from 36.83 ±1.36% to 16.38 ±1.17% and from 15.64 ±1.09% to 9.42±0.98%, respectively, as compared with the control group.
Conclusion
p21waf1gene transfection can inhibit the growth of human breast carcinoma cells and block the cell cycle, which then inhibit the genesis and development of breast cancer. So it may become a new method to treat breast cancer.
Key words:
Gene transfection; p21waf1; Cell proliferation; Breast carcinoma
Hongsheng SONG, Wei-li JIA, Rui-feng CHONG, Fu-nian LI, Xiang-ping LIU, Xin-gang WANG, Yu WANG. Effect of p21 waf1 gene transfection on the proliferation of human breast carcinoma cells[J]. Chinese Journal of Breast Disease(Electronic Edition), 2008, 02(05): 27-30.