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中华乳腺病杂志(电子版) ›› 2019, Vol. 13 ›› Issue (06) : 331 -335. doi: 10.3877/cma.j.issn.1674-0807.2019.06.003

所属专题: 文献

论著

Delta/Notch样表皮生长因子相关受体在三阴性乳腺癌中的表达及临床意义
黄红梅1, 荣欣欣1, 李静佳1, 谢少利2, 杨懿1, 周方方1, 李金穗2, 侯令密2, 邓世山1, 刘家有1,()   
  1. 1. 637000 四川 南充,川北医学院人体解剖学教研室
    2. 637000 四川 南充,川北医学院附属医院甲状腺乳腺外科
  • 收稿日期:2019-05-04 出版日期:2019-12-01
  • 通信作者: 刘家有
  • 基金资助:
    四川省教育厅创新团队资助项目(17TD0016); 川北医学院科研发展计划资助项目(CBY15-A-ZD17;CBY17-A-ZD08); 南充市科技与知识产权局市校科技战略合作专项(NSMC20170401)

Expression of Delta/Notch-like epidermal growth factor-related receptor in triple negative breast cancer and its clinical significance

Hongmei Huang1, Xinxin Rong1, Jingjia Li1, Shaoli Xie2, Yi Yang1, Fangfang Zhou1, Jinsui Li2, Lingmi Hou2, Shishan Deng1, Jiayou Liu1,()   

  1. 1. Department of Anatomy, North Sichuan Medical College, Nanchong 637000, Sichuang Province, China
    2. Department of Thyroid and Breast Surgery, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuang Province, China
  • Received:2019-05-04 Published:2019-12-01
  • Corresponding author: Jiayou Liu
  • About author:
    Corresponding author: Liu Jiayou, Email:
引用本文:

黄红梅, 荣欣欣, 李静佳, 谢少利, 杨懿, 周方方, 李金穗, 侯令密, 邓世山, 刘家有. Delta/Notch样表皮生长因子相关受体在三阴性乳腺癌中的表达及临床意义[J/OL]. 中华乳腺病杂志(电子版), 2019, 13(06): 331-335.

Hongmei Huang, Xinxin Rong, Jingjia Li, Shaoli Xie, Yi Yang, Fangfang Zhou, Jinsui Li, Lingmi Hou, Shishan Deng, Jiayou Liu. Expression of Delta/Notch-like epidermal growth factor-related receptor in triple negative breast cancer and its clinical significance[J/OL]. Chinese Journal of Breast Disease(Electronic Edition), 2019, 13(06): 331-335.

目的

探讨Delta/Notch样表皮生长因子相关受体(DNER)在三阴性乳腺癌(TNBC)中的表达情况,并分析其与患者的临床病理特征之间的关系。

方法

收集2017年3月至2018年3月川北医学院附属医院收治的39例TNBC患者的癌组织及癌旁正常组织标本进行回顾性研究,运用组织切片免疫组织化学法检测DNER蛋白表达水平,用RT-PCR检测组织中DNER mRNA表达水平。用配对χ2检验(McNemar检验)比较乳腺癌组织及癌旁正常组织中DNER阳性率的差异,用χ2检验或Fisher精确概率法分析DNER蛋白表达与患者临床病理特征之间的关系。用配对t检验比较DNER mRNA在乳腺癌组织与癌旁正常组织中的表达水平,用方差分析和独立样本t检验分析DNER mRNA表达与乳腺癌患者临床病理特征的关系,两两比较用LSD法。

结果

DNER蛋白在TNBC组织中的阳性表达率为82.1%(32/39),高于在癌旁正常组织中的阳性表达率(23.1%, 9/39)(P<0.001)。DNER蛋白在TNBC组织中的阳性表达率与肿瘤直径(χ2= 6.121,P=0.026)、淋巴结转移(P=0.005)及TNM分期(P=0.030)有关。RT-PCR检验结果显示,DNER mRNA在TNBC组织中的相对表达量高于其癌旁正常组织(0.768±0.067比0.487±0.041,t=4.793, P<0.001);DNER mRNA在TNBC组织中的表达与肿瘤直径、TNM分期及淋巴结转移有关(F=8.392, P=0.001; t=5.178,P<0.001; t=4.925,P<0.001)。

结论

DNER蛋白及mRNA的高表达与TNBC的发生、发展及转移密切相关,可能是乳腺癌诊断和治疗的潜在靶标。

Objective

To explore the expression of Delta/Notch-like epidermal growth factor-related receptor (DNER) in triple negative breast cancer (TNBC) and analyze its correlation with clinicopathological characteristics of TNBC patients.

Methods

The samples of cancer tissues and adjacent normal tissues were collected from 39 cases of TNBC in the Affiliated Hospital of North Sichuan Medical College from March 2017 to March 2018 for a retrospective study. The protein and mRNA expressions of DNER in samples were detected by immunohistochemistry and RT-PCR, respectively. The paired χ2 test (McNemar test) was used to compare the DNER-positive rates between breast cancer tissues and adjacent normal tissues. χ2 test or Fisher’s exact test was used to analyze the relationship between DNER protein expression and clinicopathological characteristics of TNBC patients. The DNER mRNA expression was compared between breast cancer tissues and adjacent normal tissues by paired t test. The correlation between DNER mRNA expression and clinicopathological characteristics was analyzed by independent-sample t test and analysis of variance. Pairwise comparison was performed using the LSD test.

Results

Immunohistochemical results showed that the positive rate of DNER protein in TNBC tissues was 82.1%(32/39), significantly higher than 23.1% (9/39) in adjacent normal tissues (P<0.001), and the positive rate of DNER protein was associated with tumor diameter (χ2= 6.121, P=0.026), TNM staging (P=0.030) and lymph node metastases (P=0.005). Results of RT-PCR showed that DNER mRNA expression in TNBC tissues was 0.768±0.067, significantly higher than 0.487±0.041 in adjacent normal tissues (t=4.793, P<0.001). The expression of DNER mRNA in TNBC tissues was correlated with tumor diameter (F=8.392, P=0.001), TNM staging (t=5.178, P<0.001) and lymph node metastases (t=4.925, P<0.001).

Conclusion

The high expression of DNER protein and mRNA is closely related to the occurrence, development and metastasis of TNBC, so DNER may be a potential target for the diagnosis and treatment of TNBC.

图1 DNER蛋白在三阴性乳腺癌组织及癌旁正常组织中的表达情况(SP ×200) a图所示DNER蛋白在三阴性乳腺癌组织中高表达;b图所示DNER蛋白在癌旁正常组织中低表达
表1 Delta/Notch样表皮生长因子相关受体蛋白在三阴性乳腺癌组织及癌旁正常组织中的表达(例)
表2 39例三阴性乳腺癌患者的临床病理特征与DNER蛋白表达的关系[例(%)]
图2 部分三阴性乳腺癌患者DNER mRNA的PCR扩增产物电泳图
表3 39例三阴性乳腺癌患者的临床病理特征与DNER mRNA表达之间的关系
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