目的

通过检测分析乳腺癌患者外周血细胞角蛋白19(cytokeratin19,CK19)及人乳腺球蛋白(hu man ma mmaglobin,h MA M)mRNA 表达,探讨其作为判断乳腺癌的相关指标。

方法

应用荧光定量PCR(Fluorescence Quantification-Pol y merase Chain Reaction,FQ-PCR)技术对40例乳腺癌患者及10名健康志愿者外周血中CK19及h MA M mRNA 的表达进行测定。运用χ² 检验和Fisher确切概率法对各组间差别进行统计学分析;乳腺癌患者CK19 和h MA M 表达相关性分析采用Kappa检验。

结果

乳腺癌患者中CK19 和h MA M 阳性率分别为52.5%(21/40)和55%(22/40),均显著高于正常对照组(CK19 阳性为1/10,h MA M 均为阴性)(P ＜0.050)。乳腺癌患者CK19 和h MAM 阳性表达具有相关性(Kappa=0.849,P=0.000)。CK19阳性表达与肿瘤大小、淋巴结转移和PR 有密切关系(P＜0.050),与ER、HER-2无关;h MA M 阳性表达与肿瘤大小、淋巴结转移、ER、HER-2 有关(P＜0.050),与PR 无关。

结论

联合检测乳腺肿瘤患者外周血中CK19及h MA M 应用于临床动态监测,可起到帮助临床诊断的作用。

Objective

To detect and anal yze t he expression of cytokeratin-19(CK19)and hu man mammaglobin (h MA M)mRNA in peripheral blood of breast cancer patients to explore t heir clinical significance.

Methods

Fl uorescence quantification-poly merase chain reaction(FQ-PCR)was used to detect the expressions of CK19 and h MA M mRNA in peripheral blood of 40 breast cancer patients and 10 healthy volunteers.Chi-square test and Fisher test were used for co mparison bet ween gr oups,and Kappa test was applied to analyze t he correlation bet ween CK19 and h MAM expressions.

Results

The positive expression rates of CK10 and h MA M in breast cancer patients were 52.5% (21/40)and 55% (22/40),respectively,significantly higher than those of the healthy controls(P ＜0.050).There was a correlation bet ween CK19 and h MA M expressions in t he breast cancer patients(Kappa=0.849,P=0.000).The positive expression of CK19 was correlated wit h tumor size,ly mph node metastasis and progesterone receptor(PR)(P＜0.050),not correlated with estrogen receptor(ER)and hu man epider mal growth factor receptor2(HER-2).The positive expression of h MAM was correlated with tumor size,ly mph node metastasis,ER and HER-2(P＜0.050),but was not correlated with PR.

Conclusions

Detecting CK19 and h MAM mRNAin peripheral blood of breast cancer patients can be used in clinical dynamic monitoring,and is helpf ul in clinical diagnosis.