Effect of tamoxifen on the growth of breast cancer MCF-7 cells transfected with exogenous ERβ1 gene

doi:10.3877/cma.j.issn.1674-0807.2008.01.011

Abstract

Abstract:

Objective

To study the effects of exogenous ERβ1 on the growth of breast cancer MCF-7 cells under different treatment with estrogen or tamoxifen.

Methods

An eukaryotic expression vector containing human entire coding sequence of ERβ (pcDNA3.1-l EGFPERβ1) was transfected into human breast cancer MCF-7 cells using lipofectin reagent. The biological activity of ERβ1was detected with the expression of ERβ1 protein by Western blot. The growth properties of MCF-7, transfected MCF-7 cells under different treatment, including E2 (17beta-lestradiol) and tamoxifen, were observed.

Results

Western blot analysis showed that the protein level of ERβ1 in the transfected MCF-7 cells was markedly increased.Exogenous ERβ1 expression inhibit the growth properties of MCF-7 cells under normal condition. The transfected MCF-7 cells proliferated at the same rate as naive cells in the presence of tamoxifen, whereas a strong inhibition of the proliferation of the transfected MCF-7 cells in the presence of E2 was observed.

Conclusions

Exogenous ERβ1 expression does not increase the resistance to tamoxifen, and a strong inhibition of the proliferation may occurre in the presence of E2.

Key words: Breast neoplasma, Estrogen receptor, Endocrine tHERapy, Gene transfection

Yan ZHOU, Jun JIANG, Xin-hua YANG, Lin-jun FAN, Yi ZHANG, Xian-chun CHEN. Effect of tamoxifen on the growth of breast cancer MCF-7 cells transfected with exogenous ERβ1 gene[J]. Chinese Journal of Breast Disease(Electronic Edition), 2008, 02(01): 36-39.

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URL: https://zhrxbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0807.2008.01.011

https://zhrxbzz.cma-cmc.com.cn/EN/Y2008/V02/I01/36

Figures/Tables 1

References 10

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细胞及分组	吸光度A值
细胞及分组	1d	2d	3d	5d	7d
MCF-7对照	0.683±0.047	0.872±0.040	1.194±0.050	1.525±0.009	1.702±0.059
MCF-7+E2	0.755±0.008	1.236±0.066	1.642±0.054	1.786±0.071	2.120±0.037
MCF-7+TAM	0.760±0.016	0.675±0.007	0.731±0.006	0.763±0.014	0.882±0.010
MCF-7^ERβ对照^a	0.480±0.058	0.699±0.035	1.059±0.106	1.407±0.099	1.515±0.107
MCF-7^ERβ+E2^b	0.758±0.016	1.157±0.047	1.391±0.079	1.536±0.059	1.873±0.064
MCF-7^ERβ+TAM	0.737±0.011	0.644±0.039	0.685±0.008	0.745±0.009	0.836±0.007

细胞及分组	吸光度A值
细胞及分组	1d	2d	3d	5d	7d
MCF-7对照	0.683±0.047	0.872±0.040	1.194±0.050	1.525±0.009	1.702±0.059
MCF-7+E2	0.755±0.008	1.236±0.066	1.642±0.054	1.786±0.071	2.120±0.037
MCF-7+TAM	0.760±0.016	0.675±0.007	0.731±0.006	0.763±0.014	0.882±0.010
MCF-7^ERβ对照^a	0.480±0.058	0.699±0.035	1.059±0.106	1.407±0.099	1.515±0.107
MCF-7^ERβ+E2^b	0.758±0.016	1.157±0.047	1.391±0.079	1.536±0.059	1.873±0.064
MCF-7^ERβ+TAM	0.737±0.011	0.644±0.039	0.685±0.008	0.745±0.009	0.836±0.007

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