Abstract:

Objective

To investigate the inhibitive efficacy of individual antisense RNA in combination with wild-type p53 gene(wt-p53) in vitro on the proliferation of breast cancer cells and explore its possible gene therapeutic implications.

Methods

pC53-SN3 plasmids were transferred into MDA-MB-231 cells and transfected WTp53-231 cells which stably expressed wt-p53 were screened with G418. MDA-MB-231 cells and Wtp53-231 cells were exposed to cationic liposome-ASp53exon8'RNA,and the growth inhibition rate,expression of p53,and induction of apoptosis were measured with MTT method,TUNE method,and immunocytochemistry staining with LSAB method respectively 48 hours later.

Results

When WTp53-231 cell was transfected with ASp53exon8'RNA,the growth inhibition of tumor cells and increase of apoptotic rate were manifested in WTp53-231 cell, furthermore,synergistic effects were observed compared with MDA-MB-231 cell.

Conclusions

ASp53exon8'RNA in combination with wt-p53 gene transfer can synergistically suppress cell growth,which may serve as effective strategy for human cancer treatment.

Key words: Breast neoplasma, Individual antisense RNA, p53 gene, Gene therapy