Abstract:
Objective
To investigate the anti-tumor effect of short hairpin RNA (shRNA)-mediated inhibition of VEGF-C (vascular endothelial growth factor C) on human breast cancer in vitro.
Methods
Breast cancer cell line MCF-7 was stably transfected with a VEGF-C short hairpin RNA(shRNA) plasmid vector labeled with green fluorescent protein. The transfected cells were visualized by fluorescent microscope and assayed by flow cytometry. The expression of VEGF-C in transfected cells was determined by RT-PCR and Western blot. The cell growth inhibition rate and the invasive capacity were evaluated by MTT method and reconstituted basement membrane invasion assay.
Results
VEGF-C shRNA specificly and effectively downregulated VEGF-C mRNA and protein expression in vitro. And it also effectively inhibited proliferation and invasive capacity of MCF-7 cells when compared to vehicle,vector and control shRNA.
Conclusions
These data in our study demonstrated that VEGF-C plays an important role in tumor growth,invasive capacity of breast cancer.RNA interference targeting VEGF-C may serve as a potential therapeutic intervention for human breast cancer.
Key words:
Vascular endothelial growth factor-C,
Breast carcinoma,
Short hairpin RNA,
RTPCR,
Western blot
Li-cheng GU, Huan-Qing XIAO, Guang-hui ZHU, Shu-hua LI. Down-regulation of VEGF-C using short hairpin RNA inhibits proliferation and invasion of human breast cancer cells[J]. Chinese Journal of Breast Disease(Electronic Edition), 2008, 02(01): 32-35.