shRNA-mediated IGF-1R silencing inhibits proliferation and migration of breast cancer cell MDA-MB-231 in vitro

doi:10.3877/cma.j.issn.1674-0807.2011.01.009

Abstract

Abstract:

Objective

To investigate the effect of short hairpin RNA（shRNA）-mediated inhibition of type I insulin-like growth factor receptor（IGF-1R）on breast cancer cell MDA-MB-231.

Methods

Breast cancer cell MDA-MB-231 was transfected with an IGF-1R shRNA plasmid vector labled with green fluorescent protein.The transfected cells were visualized by fluorescent microscope.The expression levels of IGF-1R mRNA and protein were detected respectively by RT-PCR and Western blot.The cell proliferation was evaluated by CCK-8 assay，and cell migration capacity was evaluated by transwell migration assay.Repeated measures ANOVA and multivariate analysis of variance were carried out for comparison of cell proliferation between groups，and analysis of variance was used for comparison of cell migration and the results by RT-PCR between groups.

Results

IGF-1R shRNA plasmid vector was successfully established.The transfection efficiency was 55%—60%.IGF-1R shRNA effectively down-regulated IGF-1R mRNA and protein expression.The proliferation and migration capacity of MDA-MB-231 cells were also effectively inhibited when compared to control shRNA（all P＜0.05）.

Conclusion

IGF-1R shRNA expression plasmid can effectively decrease IGF-1R gene expression，and inhibit the proliferation and migration capacity of MDA-MB-231 cell in vitro.

Key words: type I insulin-like growth factor receptor, breast neoplasms, short hairpin RNA, RNA interference

Ya-ning CHEN, Chen-fang ZHU, Yan GU. shRNA-mediated IGF-1R silencing inhibits proliferation and migration of breast cancer cell MDA-MB-231 in vitro[J]. Chinese Journal of Breast Disease(Electronic Edition), 2011, 05(01): 29-33.

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Figures/Tables 3

References 13

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组别	IGF-1R mRNA 表达量	F值	P值
IGF-1R shRNA组	0.32±0.02^a	30.596	0.01
control shRNA组	0.66±0.09^b
空白组	0.67±0.06

组别	IGF-1R mRNA 表达量	F值	P值
IGF-1R shRNA组	0.32±0.02^a	30.596	0.01
control shRNA组	0.66±0.09^b
空白组	0.67±0.06

组别	D（450）值
组别	1 d	2 d	3 d	4 d
IGF-1R shRNA组	0.20±0.02	0.23±0.02^a	0.33±0.02^a	0.46±0.04^a
control shRNA组	0.21±0.02	0.32±0.01	0.50±0.02	0.62±0.03
空白组	0.21±0.02	0.31±0.01	0.51±0.02	0.63±0.03

组别	D（450）值
组别	1 d	2 d	3 d	4 d
IGF-1R shRNA组	0.20±0.02	0.23±0.02^a	0.33±0.02^a	0.46±0.04^a
control shRNA组	0.21±0.02	0.32±0.01	0.50±0.02	0.62±0.03
空白组	0.21±0.02	0.31±0.01	0.51±0.02	0.63±0.03

组别	D（570）值	F值	P值
IGF-1R shRNA组	0.08±0.01^a	81.438	0.00
control shRNA组	0.13±0.01^b
空白组	0.13±0.01

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