目的

探讨三阴性乳腺癌患者手术前、后血清白细胞介素8(IL-8)、环氧化酶2(COX-2)表达变化及临床意义。

方法

采用酶联免疫吸附测定-双抗体夹心法(enzyme linked immunosorbent assay sandwich technique,ELISA)检测手术前、后72例乳腺癌患者(乳腺癌组)和54例良性乳腺肿物患者(对照组)血清IL-8、COX-2的表达。血清IL-8、COX-2含量在两组间比较采用t检验。术前与术后比较和术后与术后1年比较采用配对t检验。

结果

手术前乳腺癌组血清IL-8、COX-2表达明显高于对照组(P 值均是0.000)。手术后乳腺癌组与对照组比较血清IL-8、COX-2表达差异均无统计学意义(P＞0.050)。乳腺癌组手术前血清IL-8、COX-2表达明显高于手术后(P 值均是0.000)。72例乳腺癌患者中术后1年内复发4例;4例复发患者术后和术后1年血清IL-8、COX-2水平均高于无复发患者(P 值均＜0.050)。

结论

乳腺癌患者手术前、后IL-8和COX-2水平变化显著,可能对判断治疗效果有一定的参考意义。

Objective

To explore the expressions of serum interlueukin-8(IL-8)and cyclooxygenase-2(COX-2)in triple negative breast cancer patients before and after surgery and their clinical significance.

Methods

Specimens from 72 triple negative breast cancer patients(the breast cancer group)and 54 patients with benign breast mass (the control group)before and after operation were selected.The expressions of IL-8 and COX-2 in both groups before and after surgery were assessed using enzyme linked immunosorbent assay-sandwich technique(ELISA).Student's t test was used for the comparison of the expressions of IL-8 and COX-2 between the two groups,and paired t test was used for the comparison between pre-operation and post-operation.

Results

Before surgery the expression levels of IL-8 and COX-2 in the breast cancer group was markedly higher than in the control group(both P=0.000),but after operation there was no statistical difference between the two groups(both P＞0.050).The expression levels of IL-8 and COX-2 before operation were higher than after operation in the breast cancer group(both P=0.000).Among the 72 breast cancer patients,postoperative recurrence occurred in 4,and their IL-8 and COX-2 expression levels were higher than those of the nonrecurrence patients(both P＞0.050).

Conclusions

The changes of the expressions of IL-8 and COX-2 in triple negative breast cancer patients before and after operation are obvious and may be helpful to judge therapeutic effect.