目的

探讨P-糖蛋白(P-gp)和肺耐药蛋白(LRP)在浸润性乳腺癌中的表达情况及其临床意义。

方法

采用搓网法获取乳腺癌手术切除标本制成的完整单细胞悬液,再用流式细胞技术对40例乳腺癌组织及相应癌旁组织中P-gp、LRP的表达情况进行定量分析。

结果

乳腺癌组织P-gp、LRP表达与相应癌旁组织比较,差异均有统计学意义(P-gp:t=-7.777,LRP:t=-9.861,P 均＜0.050)。在不同年龄、肿瘤大小、病理类型、临床分期、分化程度和雌、孕激素受体状态的患者间,两种耐药相关蛋白的表达差异均无统计学意义(P ＞0.050)。有淋巴结转移组P-gp表达与无淋巴结转移组比较,差异无统计学意义(t=0.102,P=0.919)。有淋巴结转移组LRP的表达为(41.2±18.5)%,无淋巴结转移组为(30.1±15.4)%,有淋巴结转移组LRP表达显著高于无淋巴结转移组(t=2.074,P=0.045)。乳腺癌组织P-gp与LRP的表达间存在正相关性(r=0.698,P＜0.001)。

结论

本研究结果可为寻找浸润性乳腺癌的有效辅助化疗药物提供参考。

Objective

To investigate the expressions of P-glycoprotein(P-gp)and lung resistance protein (LRP)in breast carcinoma tissues and their clinical significance.

Methods

Single-cell suspension with intact cellular membrane taken from breast cancer resection specimens were prepared using rubbing-net technique and flow cytometry(FCM)was used to quantitatively examine the expressions of P-gp and LRP in carcinoma tissues and corresponding peri-carcinoma tissues in 40 breast cancer cases.

Results

The expressions of P-gp and LRP in breast cancer tissues were statistically different from those of the corresponding peri-carcer tissues(P＜0.050).No statistically significant difference existed in the P-gp and LRP expressions of the tumor cells in age,tumor size,pathological types,clinical stages and cell differentiation degree,and between negative and positive expressions of the estrogen and progestin receptors of the patients(P＞0.05).There was no statistical difference in P-gp expression between the lymph node metastasis negative and positive patients(t=0.102,P=0.919).The expression of LRP was higher in the lymph node metastasis patients(41.2±18.5)%than that in the non-metastasis patients(30.1±15.4)% (t=2.074,P=0.045).There was a positive correlation between the expression of P-gp and the expression of LRP(r=0.698,P＜0.001).

Conclusions

The results of this study provides references in looking for effective chemotherapy drugs for invasive breast cancer.