目的

研究浸润性T淋巴细胞和Th细胞因子在不同类型乳腺癌中表达的意义。

方法

利用免疫组织化学链霉亲和素生物素法(labelled streptavidin-biotin method,LSAB)方法检测61例浸润性微乳头状癌(invasive micropapillary carcinoma,IMPC)、71例浸润性导管癌(invasive ductal carcinoma,IDC)和27例髓样癌(medullary carcinoma,MC)3种乳腺癌中浸润T淋巴细胞亚群分布以及Th细胞因子的表达。T淋巴细胞组间比较采用Kruskal-Wallis Test,组内比较采用Wilcoxon Signed Ranks Test;组间Th细胞因子的比较采用χ²检验。

结果

CD3、CD8和CD4均表达于淋巴细胞胞膜。CD3⁺T细胞在3组间差异无统计学意义(P=0.735)。CD8⁺T和CD4⁺T细胞在3组间差异均有统计学意义(P=0.000,P=0.012)。Th细胞因子主要表达于肿瘤细胞和淋巴细胞的胞质。IL-2表达在IMPC组最低(25/61,41.0%),其次是IDC组(33/71,46.5%),最高是MC组(20/27,74.1%),3组间差异有统计学意义(P=0.014);IFN-γ的表达类似于IL-2,IMPC组最低(39/61,63.9%),其次是IDC组(57/71,80.3%),最高是MC组(24/27,88.9%),3组间差异有统计学意义(P=0.019);IL-4的表达在IDC组最高(42/71,59.2%),其次是IMPC组(19/61,31.1%),最低是MC组(5/27,18.5%),3组间差异有统计学意义(P=0.000);IL-10的阳性率在IMPC组是87.8%(47/61),在IDC组是87.3%(62/71),在MC组是88.9%(24/27),3组间差异无统计学意义(P＞0.050)。

结论

浸润性T淋巴细胞和Th细胞因子在不同类型乳腺癌中表达不同。

Objective

To study the expression of tumor-infiltrating lymphocytes(TILs)and T helper cytokines in invasive micropapillary carcinoma(IMPC),invasive ductal carcinoma(IDC)and medullary carcinoma(MC)of the breast.

Methods

Three T-cell markers including pan-T cell marker(CD3),T-helper cell marker(CD4),cytotoxic T cell marker(CD8),and four T-helper cytokines including interleukin-2(IL-2),interferon-gamma(IFNγ),interleukin-4(IL-4),and interleukin-10(IL-10)were tested immunohistochemically in the three groups,(IMPC n=61,IDC n=71,and MC n=27)on formalin-fixed,paraffin embedded tumors.Kruskal-Wallis Test and Wilcoxon Signed Ranks Test were used for analysis of TILs;chi-square test was used for analysis of T helper cytokines.

Results

Markers,CD3,CD4 and CD8,all expressed in the cell membrane of lymphocytes.There was no statistical difference in CD3⁺T cells number between the three groups(P=0.705);CD8⁺T cells and CD4⁺T cells were statistically different between the three groups(P=0.000,P=0.012).T helper cytokines expressed in cytoplasm of tumour cells and lymphocytes.The expression of IL-2 was the highest in the MC group(20/27,74.1%),followed by the IDC group(33/71,46.5%),and the lowest in the IMPC group(25/61,41.0%),there was a statistical difference between the three groups(P=0.014).The expression of IFN-γwas the highest in the MC group(24/27,88.9%)followed by the IDC group(57/71,80.3%),and the lowest in the IMPC group(39/61,63.9%),there was a statistical difference between the three groups(P=0.019).The positive expression of IL-4 was the highest in the IDC group(42/71,59.2%),followed by the IMPCgroup(19/61,31.1%)and the lowest in the MC group(5/27,18.5%),there was a statistical difference between the three groups(P=0.000).The positive rate of IL-10 expression was 87.8%(47/61)in the IMPC group,87.3%(62/71)in the IDC group,and 88.9%(24/27)in the MC group,with no statistical difference between the three groups.(P＞0.050).

Conclusion

The expressions of infiltrating T lymphocytes and T helper cytokines were different in different types of breast carcinomas,which may be responsible for different biologic behavior.