乳腺浸润性微乳头状癌中CD44+/CD24-/low和CD24+表型细胞的研究

doi:10.3877/cma.j.issn.1674-0807.2009.05.010

中华乳腺病杂志(电子版) ›› 2009, Vol. 03 ›› Issue (05) : 524 -534. doi: 10.3877/cma.j.issn.1674-0807.2009.05.010

实验研究

乳腺浸润性微乳头状癌中CD44+/CD24-/low和CD24+表型细胞的研究

李伟东¹, 刘芳芳¹, 徐新生¹, 刘冰冰¹, 崔力方¹, 位嘉¹, 郭晓静¹, 郎荣刚¹, 范宇¹, 谷峰¹, 付丽¹^,^†(

)

1.300060 天津,天津医科大学附属肿瘤医院乳腺病理科教育部乳腺癌防治重点实验室

收稿日期:2009-06-19 出版日期:2009-10-01
通信作者: 付丽
基金资助:
教育部长江学者和创新团队发展计划资助项目(IRT0743)国家自然科学基金资助项目(30600225)国家“863”计划项目(2006 AA02 A249)国家“973”计划资助项目(2009CB521700)

Study on CD44⁺/CD24^-/low and CD24⁺tu mor cells in invasive micropapillary carcinoma of the breast

Wei-dong LI¹, Fang-fang LIU¹, Xin-sheng XU¹, Bing-bing LIU¹, Li-fang CUI¹, Jia WEI¹, Xiao-jing GUO¹, Rong-gang LANG¹, Yu FAN¹, Feng GU¹, Li FU^†^,¹()

1.Department of Breast Cancer Pathology and Research Laboratory,Cancer Institution and Hospital,Tianjin Medical University,Tianjin 300060,China

Received:2009-06-19 Published:2009-10-01
Corresponding author: Li FU

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引用本文：

李伟东, 刘芳芳, 徐新生, 刘冰冰, 崔力方, 位嘉, 郭晓静, 郎荣刚, 范宇, 谷峰, 付丽. 乳腺浸润性微乳头状癌中CD44+/CD24-/low和CD24+表型细胞的研究[J/OL]. 中华乳腺病杂志(电子版), 2009, 03(05): 524-534.

Wei-dong LI, Fang-fang LIU, Xin-sheng XU, Bing-bing LIU, Li-fang CUI, Jia WEI, Xiao-jing GUO, Rong-gang LANG, Yu FAN, Feng GU, Li FU. Study on CD44⁺/CD24^-/low and CD24⁺tu mor cells in invasive micropapillary carcinoma of the breast[J/OL]. Chinese Journal of Breast Disease(Electronic Edition), 2009, 03(05): 524-534.

目的

研究乳腺浸润性微乳头状癌(invasive micropapillary carcinoma,IMPC)的干细胞表型,从干细胞和上皮间质转化(epithelial-mesenchy mal transition,EMT)角度探讨I MPC高侵袭、高转移恶性生物学行为的原因。

方法

选取术前未经放化疗治疗患者的I MPC 82 例和乳腺非特殊型浸润性导管癌(invasive ductal carcinoma not other wise specified,IDC-NOS)80例石蜡包埋组织标本切片,通过免疫组织化学双染技术检测两组肿瘤组织中CD44⁺/CD24^-/low(CD24^-)和CD24⁺的表达、定位和分布情况,并分析其与各临床病理学特征之间的关系。定量资料采用Student's t检验,定性资料采用Mann-Whitney U 检验、Kruskal-Wallis检验、χ² 检验或校正χ² 检验。两组之间相关性采用Spear man's秩相关分析。

结果

(1)I MPC组肿瘤细胞的CD44⁺/CD24^-/low阳性表达率(48.8%,40/82例),明显高于IDC-NOS组(31.3%,25/80例)(χ²=5.180,P=0.023)。(2)53.7%(44/82例)的I MPC微细间质组织内见单个散在的CD44⁺/CD24^-/low肿瘤细胞,且该细胞免疫组织化学染色Vi mentin及α-SMA 阳性,E-Cadherin阴性。IDC-NOS间质内罕见CD44⁺/CD24^-/low肿瘤细胞。(3)I MPC微乳头结构中CD44⁺/CD24^-/low与间质内的CD44⁺/CD24^-/low阳性表达细胞呈明显正相关(r=0.516,P＜0.001),并且I MPC微乳头结构及间质中CD44⁺/CD24^-/low阳性表达在有无淋巴管侵犯和有无淋巴结外软组织浸润方面的差异均有统计学意义(P＜0.050),即有淋巴管侵犯及淋巴结外软组织浸润者CD44⁺/CD24^-/low阳性表达率较高。(4)I MPC组中CD24⁺细胞阳性表达率79.3%(65/82例),明显高于IDC-NOS组(60.0%,48/80例)(χ²=7.126,P=0.008),且I MPC中淋巴结转移阳性组CD24 的表达高于阴性组,差异有统计学意义(χ²=8.834,P=0.003)。

结论

I MPC肿瘤细胞中干细胞的存在及上皮间质转化可能是I MPC高淋巴管侵犯、高淋巴结转移及耐药等恶性生物学行为的重要原因。研发针对乳腺癌干细胞的药物也可作为治疗乳腺癌的一个方法。

关键词: 乳腺肿瘤, 乳腺浸润性微乳头状癌, 干细胞, CD44⁺/CD24^-/l^ow, CD24⁺, 上皮间质转化

Objective

To study the stem cell phenotype of invasive micr opapillary carcino ma(I MPC)of the breast.Through the stem cell and epithelial-mesenchy mal transition,the causes of biological behaviour of high metastasis and invasion of IMPC was investigated.

Methods

The expression,l ocation and distribution of CD44 and CD24 were deter mined by immunohistochemical double staining in 82 cases of IMPC and 80 cases of invasive ductal carcino ma not other wise specified (IDC-NOS),under going without preoperative chemot herapy and radiot herapy, and their relationship with clinicopat hological feat ures was anal yzed.St udent'st test was used for quantitative data.Mann-Whitney Utest,Kruskal-Wallis test andχ² test or revisedχ² test were used for qualitative data.Spear man's test was used for association bet ween groups.

Results

The positive expression rate of CD44⁺/CD24^-/low tumor cells was higher in the IMPC gr oup(48.8%,40/82 cases)t han in the IDC-NOS gr oup(31.3%,25/80 cases)(χ²=5.180,P=0.023).The CD44⁺/CD24^-/low tu mor cells was also detected in adjacent stro ma surrounding the micropapillary structure in 53.7%(44/82 cases)of I MPC,with Vi mentin andα-SMA positive and E-Cadherin negative,but scarce in stro ma of IDC-NOS.(3)The CD44⁺/CD24^-/low tu mor cells in micr opapillar y str ucture of IMPC was positively associated with that instroma(r=0.516,P＜0.001),moreover,they were bot h associated withly mphovascular invasion and extranodal extension respectively,and there was statistical difference between lymphovascular invasion and extranodal extension(P＜0.050),that is the positive expression rate of CD44⁺/CD24^-/l^ow in ly mphovascular invasion and extranodal extension was high.(4)The positive expression rate of CD24⁺t u mor cells was also higher in IMPC (65/82 cases)than in IDC-NOS(48/80 cases)(χ²=7.126,P=0.008),and in IMPC the expression of CD24⁺ was higher in positively mphnode metastasis than in negative,with statistical difference(χ²=8.834,P=0.003).

Conclusions

The existence of stem cells and epithelial-mesenchy mal transition in IMPC tumor cells may play an important rolein aggressiveness and higher metastatic risk of IMPC of the breast.It can be a method to develop drugs targeting breast cancer stemcells.

Key words: Breast neoplasms, IMPC, Stem cells, CD44⁺/CD24^-/low, CD24⁺, Epithelial-mesenchy mal transition

表1 I MPC 和IDC-NOS临床病理学特征的比较

分组	年龄(岁)	肿瘤大小(cm)	组织学分级(例)			LNM(例)		pTNM分期(例)			ENE(例)		LVI(例)		CD24^-(例)		CD24⁺(例)
分组	年龄(岁)	肿瘤大小(cm)	Ⅰ	Ⅱ	Ⅲ	-	+	Ⅰ	Ⅱ	Ⅲ～Ⅳ	-	+	-	+	-	+	-	+
IMPC	54.79±11.17	3.20±1.46	10	48	24	7	75	4	31	47	30	52	24	58	42	40	17	65
IDC-NOS	51.99±9.95	2.58±1.16	7	59	14	37	43	10	52	18	65	15	69	11	55	25	32	48
统计量	1.686	3.000	1.079			29.112		19.958			33.308		53.772		5.180		7.126
P值	0.094^a	0.003^a	0.299^b			0.000^c		0.000^b			0.000^c		0.000^c		0.023^c		0.008^c

表1 I MPC 和IDC-NOS临床病理学特征的比较

分组	年龄(岁)	肿瘤大小(cm)	组织学分级(例)			LNM(例)		pTNM分期(例)			ENE(例)		LVI(例)		CD24^-(例)		CD24⁺(例)
分组	年龄(岁)	肿瘤大小(cm)	Ⅰ	Ⅱ	Ⅲ	-	+	Ⅰ	Ⅱ	Ⅲ～Ⅳ	-	+	-	+	-	+	-	+
IMPC	54.79±11.17	3.20±1.46	10	48	24	7	75	4	31	47	30	52	24	58	42	40	17	65
IDC-NOS	51.99±9.95	2.58±1.16	7	59	14	37	43	10	52	18	65	15	69	11	55	25	32	48
统计量	1.686	3.000	1.079			29.112		19.958			33.308		53.772		5.180		7.126
P值	0.094^a	0.003^a	0.299^b			0.000^c		0.000^b			0.000^c		0.000^c		0.023^c		0.008^c

图1 乳腺癌组织中CD44和CD24的表达 a:箭头所示IDC-NOS中CD44⁺/CD24^-/low表型肿瘤细胞,而CD24阴性表达(×200);b:箭头所示IMPC微乳头结构中的CD44⁺/CD24^-/low表型肿瘤细胞(×200);c:箭头所示IMPC微乳头结构周围间质组织中的CD44⁺/CD24^-/low表型肿瘤细胞(×200);d:箭头所示IMPC微乳头结构周围间质组织中的CD44⁺/CD24^-/low表型肿瘤细胞,与此相反,相邻的IMPC微乳头结构中的肿瘤细胞为CD24⁺表型(×400);CD44以AP-red显色,红色为阳性表达;CD24以DAB显色,棕色为阳性表达。

图2 免疫组织化学染色显示I MPC间质组织中肿瘤细胞E-CD 和Vi mentin的表达 a:E-CD 在I MPC 微乳头状结构与间质相接的外侧面低表达或表达缺失,而微乳头中间部分的肿瘤细胞间正常表达或高表达;I MPC微乳头周围间质组织中少数单个散在的肿瘤细胞可见E-CD 呈阴性表达(箭头所示)(SP ×200);b:I MPC微乳头周围间质中单个散在的肿瘤细胞与其周围其他间质细胞相同,显示Vi mentin阳性表达(箭头所示),而微乳头结构中的肿瘤细胞团却与之相反,显示Vi mentin阴性表达(SP ×200)。

表2 IMPC间质和微乳头结构中CD44⁺/CD24^-/low(CD24^-)细胞的关系

间质中CD24^-细胞	微乳头结构中CD24^-细胞(例)		r值	P值
间质中CD24^-细胞	-	+	r值	P值
-	30	8
+	12	32	0.516	0.000

表2 IMPC间质和微乳头结构中CD44⁺/CD24^-/low(CD24^-)细胞的关系

间质中CD24^-细胞	微乳头结构中CD24^-细胞(例)		r值	P值
间质中CD24^-细胞	-	+	r值	P值
-	30	8
+	12	32	0.516	0.000

表3 I MPC 临床病理学特征与CD44⁺/CD24^-/low和CD24⁺肿瘤细胞的关系

临床病理学特征	CD44⁺/CD24^-/low				CD24⁺
临床病理学特征	阴性(例)	阳性(例)	统计量	P值	阴性(例)	阳性(例)	统计量	P值
年龄
≤55	18	22			10	30
＞55	24	18	-1.093	0.274^a	7	35	-0.925	0.355^a
肿瘤大小(cm)
≤2	9	7			5	11
2～5	29	31			12	48
＞5	4	2	0.000	1.000^b	0	6	2.402	0.121^b
组织学分级
Ⅰ	7	3			0	10
Ⅱ	23	25			10	38
Ⅲ	12	12	0.471	0.493^b	7	17	2.995	0.083^b
淋巴结转移
-	3	4			5	2
+	39	36	0.005	0.946^c	12	63	8.834	0.003^c
pTNM分期
Ⅰ	2	2			2	2
Ⅱ	14	17			6	25
Ⅲ～Ⅳ	26	21	0.656	0.418^b	9	38	0.423	0.515^b
ER
-	13	13			3	23
+	29	27	0.023	0.880^d	14	42	1.958	0.162^d
PR
-	13	7			3	17
+	29	33	2.010	0.156^d	14	48	0.529	0.467^d
HER-2
-～+	32	33			14	51
++～+++	10	7	0.496	0.481^d	3	14	0.124	0.725^d

表3 I MPC 临床病理学特征与CD44⁺/CD24^-/low和CD24⁺肿瘤细胞的关系

临床病理学特征	CD44⁺/CD24^-/low				CD24⁺
临床病理学特征	阴性(例)	阳性(例)	统计量	P值	阴性(例)	阳性(例)	统计量	P值
年龄
≤55	18	22			10	30
＞55	24	18	-1.093	0.274^a	7	35	-0.925	0.355^a
肿瘤大小(cm)
≤2	9	7			5	11
2～5	29	31			12	48
＞5	4	2	0.000	1.000^b	0	6	2.402	0.121^b
组织学分级
Ⅰ	7	3			0	10
Ⅱ	23	25			10	38
Ⅲ	12	12	0.471	0.493^b	7	17	2.995	0.083^b
淋巴结转移
-	3	4			5	2
+	39	36	0.005	0.946^c	12	63	8.834	0.003^c
pTNM分期
Ⅰ	2	2			2	2
Ⅱ	14	17			6	25
Ⅲ～Ⅳ	26	21	0.656	0.418^b	9	38	0.423	0.515^b
ER
-	13	13			3	23
+	29	27	0.023	0.880^d	14	42	1.958	0.162^d
PR
-	13	7			3	17
+	29	33	2.010	0.156^d	14	48	0.529	0.467^d
HER-2
-～+	32	33			14	51
++～+++	10	7	0.496	0.481^d	3	14	0.124	0.725^d

表4 I MPC 间质和微乳头结构中CD44⁺/CD24^-/low细胞与淋巴管侵犯及淋巴结外软组织浸润的关系

病理因素	间质中CD44⁺/CD24^-/low表型肿瘤细胞				微乳头结构中CD44⁺/CD24^-/low表型肿瘤细胞
病理因素	阴性(例)	阳性(例)	χ ²值	P值	阴性(例)	阳性(例)	χ ²值	P值
淋巴管侵犯
-	19	5			19	5
+	19	39	14.703	0.000	23	35	10.607	0.001
淋巴结外软组织浸润
-	19	11			22	8
+	19	33	5.493	0.019	20	32	9.259	0.002

表4 I MPC 间质和微乳头结构中CD44⁺/CD24^-/low细胞与淋巴管侵犯及淋巴结外软组织浸润的关系

病理因素	间质中CD44⁺/CD24^-/low表型肿瘤细胞				微乳头结构中CD44⁺/CD24^-/low表型肿瘤细胞
病理因素	阴性(例)	阳性(例)	χ ²值	P值	阴性(例)	阳性(例)	χ ²值	P值
淋巴管侵犯
-	19	5			19	5
+	19	39	14.703	0.000	23	35	10.607	0.001
淋巴结外软组织浸润
-	19	11			22	8
+	19	33	5.493	0.019	20	32	9.259	0.002

[1]	Marx J.Cancer research.Mutant stem cells may seed cancer.Science,2003,301:1308-1310.
[2]	Chen L,Fan Y ,Lang R G et al.Breast carcino ma with micropapillary features:clinicopathologic study and long-ter mfollow-up of 100 case.Int J Surg Pathol,2008,16:155-163.
[3]	Guo X J,Chen L,Lang R G ,et al.Invasive micro-papillary carcinoma of the breast:association of pathologic features with ly mph node metastasis.Am J Clin Pathol,2006,126:740-746.
[4]	Sinaunkgul S,Tavassoli F A.Invasive micropapillar y carcino ma of the breast.Mod Pat hol,1993,6:660-662.
[5]	Middeton L P,Tressera F,Sobel M E,et al.Infiltrating micr opapillary carcino ma of the breast.Mod Pathol,1999,12:499-504.
[6]	Tsu magari K,Sakamoto G,Akiyama F,et al.The pathological diagnosis and clinical significance of invasive micr opapillar y carcino ma of t he breast.Jpn J Breast Cancer,2001,16:441-447.
[7]	Al Hajj M,Wicha M S,Benito H A,et al.Prospective Identification of tu morigenic breast cancer cells.Pr oc Natl Acad Sci USA,2003,100:3983-3988.
[8]	Ponti D,Costa A,Zaffaroni N,et al.Isolation and in vitro propagation of tu morigenic breast cancer cells with stem/progenitor cell properties.Cancer Res,2005,65:5506-5511.
[9]	Sheridan C,Kishi moto H,Fuchs R K,et al.CD44⁺/CD24^-breast cancer cells exhibit enhanced invasive properties:an early step necessary f or metastasis.Breast Cancer Res,2006,8:R59.
[10]	hillips T M,Mc Bride W H,Pajonk F.The response of CD44⁺/CD24^-/low breast cancer-initiating cells to radiation.J Natl Cancer Inst,2006,98:1777-1785.
[11]	braha m B K,Fritz P ,Mc Clellan M,et al.Prevalence of CD44⁺/CD24^-/low cells in breast cancer may not be associated with clinical outco me but may favor distant metastasis.Clinical Cancer Res,2005,11:1154-1159.
[12]	hristiansen J J,Rajasekaran A K.Reassessing epithelial-mesenchy mal transition as a prerequisite f or carcino ma invasion and metastasis.Cancer Res,2006,66:8319-8326.
[13]	an Y,Lang R G,Wang Y,et al.Relationship bet ween expression of cell adhesion molecules and metastatic potential in invasive micr opapillar y carcino ma of breast.Chin J Pat hol,2004,33:308-311.
[14]	ani S A,Guo W ,Liao M J,et al.The epit helial-mesenchy mal transition generates cells wit h pr operties of ste m cell.Cell,2008,133,704-715.
[15]	orel A P,Lièvre M,Tho mas C,et al .Generation of breast cancer stem cells through epit helial-mesenchy mal transition.http:// www.plosone.or g/article/inf o:doi/10.1371/jour nal.pone.0002888.
[16]	avassoli F A,Devilee P.Pathology and genetics of tu mors of the breast and female genital or gans. Worl d Healt h Or ganization Classification of Tu mors. Lyon:IARC Press,2003:35-36.
[17]	lston C W,Ellis I O.Pathological prognostic factors in breast cancer.I.The value of histological grade in breast cancer:experience fro malarge study with long-ter mfollow-up.Histopathology,1991,19:403-410.
[18]	ei J,Cui L F,Liu F F ,et al.E-selectin and sialyl lewis X expression is associated with ly mph node metastasis of invasive micropapillar y carcino ma of the breast.Inter J Sur g Pathol,2008,8:1-8.
[19]	abriella H,Par Ola B,Mar kus R,et al.The CD44⁺/CD24^-/low phonotype is enriched in basallike breast t u mors.Breast Cancer Res,2008,10:R53.
[20]	illes L E,Weiss man I L.Cancer stem cells in solid tu mors.Curr Opin Biotechnol,2007,18:460-466.
[21]	ar ol S,Hir o mitsu K,Robyn K F,et al.CD44⁺/CD24^-breast cancer cells exhibit enhanced invasive pr operties:an earl y step necessar y f or metastasis.Breast Cancer Res,2006,8:R59.
[22]	leni M ,Ioanna G,Emmanouil F,et al.The clinico-pathologic and prognostic significance of CD44⁺/CD24^-/low and CD44^-/CD24⁺tu mor cells in invasive breast carcino mas.Hu man Pathol,2008,39:1096-1102.
[23]	hier y J P.Epithelial-mesenchy mal transitions in devel op ment and pathologies.Curr Opin Cell Biol,2003,15:740-746.
[24]	ay R,Rosten P M,Hu mphries R K.CD24,a signal transducer modulating B cell activation responses,is a ver y short peptide wit h a glycosyl phosphatidylinositol me mbrane anchor.J Immunol,1991,147:1412-1416.
[25]	au mann P,Cre mers N,Kr ocese F,et al.CD24 expression causes t he acquisition of multiple cellular properties associated with tu mor growth and metastasis.Cancer Res,2005,65:10 783-10 793.
[26]	ristiansen G,Winzer K J,Mayordo mo E,et al.CD24 expression is a new prognostic marker in breast cancer.Clinical Cancer Res,2003,9:4906-4913.

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Study on CD44⁺/CD24^-/low and CD24⁺tu mor cells in invasive micropapillary carcinoma of the breast

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Study on CD44+/CD24-/low and CD24+tu mor cells in invasive micropapillary carcinoma of the breast

Study on CD44⁺/CD24^-/low and CD24⁺tu mor cells in invasive micropapillary carcinoma of the breast