目的

探讨黄芪注射液作用于basal-like型乳腺癌MDA-MB-231细胞株后对其增殖和凋亡的影响。

方法

将实验细胞分为对照组和5种不同剂量的黄芪注射液作用组。用MTT 法检测细胞增殖,流式细胞仪分析细胞周期,碘化丙啶染色检测细胞凋亡率。黄芪注射液作用于MDA-MB-231细胞48 h和72 h的OD 值采用多个均数比较的方差分析;48 h时细胞周期各阶段细胞比率的比较用χ²检验。

结果

作用48 h时,各剂量组均可抑制MDA-MB-231细胞增殖(P＜0.01),其效应呈质量浓度依赖性。作用48 h时,高剂量组(2×10^-1～2×10^-2 g/ml)黄芪注射液还可促进细胞凋亡(χ²=8.01,P=0.00);作用72 h时,高剂量组仍呈抑制作用,但随质量浓度的降低,抑制作用减少,低剂量黄芪组(2×10^-4～2×10^-5 g/ml)有促进增殖作用(P＜0.01);中高剂量组(2×10^-3 g/ml)可改变细胞周期的分布。

结论

黄芪注射液可抑制MDA-MB-231细胞增殖,并诱导其凋亡,可能为basal-like乳腺癌的治疗带来益处。

Objective

To investigate the effects of Astragalus injection on proliferation and apoptosis of basal-like breast cancer cell line MDA-MB-231.

Methods

There were six groups used in this study:one control group(cell culture without Astragalus injection)and five experimental groups in which MDAMB-231 cells were cultured with Astragalus injection at doses of(2×10^-1 g/ml),(2×10^-2 g/ml),(2×10^-3 g/ml),(2×10^-4 g/ml),and(2×10^-5 g/ml),respectively.Proliferation of MDA-MB-231 cells were evaluated with MTT assay,and cell apoptotic rate and cell cycle were measured with flow cytometry.The OD values of Astragalus injection acting on MDA-MB-231 cells for 48 and 72 hours were determined using variance analysis;chi-square test was used for the comparison of cell proportion in each cell cycle phase at 48 hours among all groups.

Results

At 48 hour coculture,in all the experimental groups the cell proliferation was inhibited(P＜0.01),which showed a dose dependence;and cell apoptosis was accelerated at the high dosage group(2×10^-1～2×10^-2 g/ml)(χ²=8.01,P=0.00).At 72 hour coculture,the inhibiting effect of Astragalus injection still maintained at the high dosage group,but reduced with the concentration degrade;MDA-MB-231 cell proliferation was increased at the low dosage group(2×10^-4～2×10^-5 g/ml)(P＜0.01).

Conclusion

Astragalus injection can inhibit MDA-MB-231 cell growth and accelerate its apoptosis.It may benefit basal-like breast cancer patients.