Abstract:
Objective
To evaluate the clinical significance of SBEM (small breast epithelial mucin) mRNA as a micrometastases marker in peripheral blood of breast cancer.
Methods
The SBEMmRNA level in peripheral blood was detected in 22 patients with gastric cancer,16 colon/rectal carcinoma,107 breast cancer,40 breast fibroadenoma and 27 healthy volunteers as controls using rested reverse transcription-polymerase chain reaction (Nested-RT-PCR).
Results
The positive expression rate of SBEMmRNA in peripheral blood of breast cancer patients was 53.27%(57/107 cases),and no positive SBEMmRNA was found in other controls.There was a significant difference between the two groups (P ＜0.05).SBEM-mRNA expression was correlated with axillary lymph nodes status and TNM (P ＜0. 01), but uncorrelated with other clinical parameters (age,tumor size,histopathlogy types,estrogen receptor and progesterone receptor).
Conclusion
SBEM-mRNA is sensitive and specific for breast cancer,and the positive expression in peripheral blood may be a marker in detecting micrometastases and judging prognosis.
Key words:
Breast carcinorna,
Micrometastasis,
Nested-RT-PCR,
SBEM-mRNA
Lei ZHONG, Jian-guo ZHANG, Bao-liang GUO, Xue-wei YANG, Ming SHAN. Detection of SBEM-mRNA level in peripheral blood of patients with breast cancer and its clinical significance[J]. Chinese Journal of Breast Disease(Electronic Edition), 2008, 02(01): 24-27.