Expression of microRNA-34c-3p in invasive breast cancer and correlation with BRCA gene

doi:10.3877/cma.j.issn.1674-0807.2019.06.002

Abstract

Abstract:

Objective

To analyze the correlation between microRNA-34c-3p (miR-34c-3p) expression and BRCA gene mutation in invasive breast cancer.

Methods

The patients treated in the Xijing Hospital, Air Force Medical University from February 2015 to June 2017 were continuously enrolled for a prospective study. The subjects were divided into the invasive breast cancer group (group A, 55 cases), the precancerous lesion group (group B, 46 cases) and the normal control group (group C, 50 cases) according to the criteria of inclusion. The expression of miR-34c-3p was detected in all tissue and blood samples. The expression of miR-34c-3p was compared among three groups using one-way analysis of variance, and pairwise comparison was performed using the LSD method. Spearman rank correlation was used to analyze the correlation between miR-34c-3p expression in invasive breast cancer tissue and blood samples and BRCA gene.

Results

(1) In the breast tissue samples, the expression of miR-34c-3p was 0.76±0.29, 1.24±0.33 and 1.62±0.36 in group A, B and C, respectively, indicating a significant difference among three groups(F=27.373, P=0.020). The miR-34c-3p expression in group A was significantly lower than that in group B or C (P= 0.024, 0.001). There was no significant difference between group B and group C (P= 0.065). (2) In the blood samples, the expression of miR-34c-3p was 0.32±0.18, 0.71±0.23 and 0.93±0.27 in group A, B and C, respectively, indicating a significant difference among three groups (F=29.534, P=0.018). The expression of miR-34c-3p in group A was significantly lower than that in group C (P=0.006). But there was no significant difference between group A and B, between group B and group C (P=0.076, 0.102). (3) The expression of miR-34c-3p was negatively correlated with the risk of BRCA gene mutation in breast cancer tissue (r=-0.548, P<0.001) and in blood samples (r=-0.312, P=0.006).

Conclusion

The low expression of miR-34c-3p is observed in invasive breast cancer tissues, which is associated with BRCA gene mutation.

Key words: MicroRNAs, Breast neoplasms, Genes, BRCA

Jiang Wu, Wenlong Shi, Qingjie Meng, Ge Zhao, Jing Fan, Ting Wang, Nanlin Li, Rui Ling. Expression of microRNA-34c-3p in invasive breast cancer and correlation with BRCA gene[J]. Chinese Journal of Breast Disease(Electronic Edition), 2019, 13(06): 326-330.

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Figures/Tables 5

References 23

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BRCA基因突变分类	例数	miR-34c-3p表达(±s)
1类	0	?
2类	3	0.73±0.22^a
3类	8	0.75±0.28
4类	19	0.76±0.26
5类	25	0.78±0.34^b
F值	?	5.328
P值	?	<0.001

BRCA基因突变分类	例数	miR-34c-3p表达(±s)
1类	0	?
2类	3	0.73±0.22^a
3类	8	0.75±0.28
4类	19	0.76±0.26
5类	25	0.78±0.34^b
F值	?	5.328
P值	?	<0.001

BRCA基因突变分类	例数	miR-34c-3p表达(±s)
1类	0	?
2类	4	0.30±0.12
3类	11	0.31±0.21
4类	17	0.31±0.16
5类	21	0.33±0.14^a
F值	?	4.263
P值	?	<0.001

BRCA基因突变分类	例数	miR-34c-3p表达(±s)
1类	0	?
2类	4	0.30±0.12
3类	11	0.31±0.21
4类	17	0.31±0.16
5类	21	0.33±0.14^a
F值	?	4.263
P值	?	<0.001

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