Expressions of miR-206 and Cx43 in primary breast tumours and metastatic lymph nodes

doi:10.3877/cma.j.issn.1674-0807.2011.01.012

Abstract

Abstract:

Objective

To explore the expression and regulation role of microRNA-206（miR-206）and connexin 43（Cx43）in primary breast tumours（PTs）and metastatic lymph nodes（MLNs）.

Methods

Eight patients of pathologically confirmed infiltrating duct carcinoma of the breast were enrolled.Pericancer normal tissues，primary breast tumors（PTs），paired normal axillary lymph nodes（NLNs）and paired metastatic lymph nodes（MLNs）were obtained from the resected tumors of the patients.The expressions of mRNA and protein of miR-206 and Cx43 in pericancer normal tissue，PTs，NLNs and MLNs were tested using real-time PCR and Western blot techniques.Comparison between groups was performed using one way analysis of variances and SNK or LSD test.

Results

Compared with the normal breast tissues，the mRNA expression of miR-206 in PTs and NLNs decreased significantly，and it further decreased in MLNs compared with PTs and NLNs（P＜0.05）.The mRNA expressions of Cx43 was significantly decreased in PTs，NLNs and MLNs compared with the normal（P＜0.05），but there was no statistical difference between PTs and MLNs（P＞0.05）.The protein expression of Cx43 in PTS and NLNs were significantly lower than in the normal（P＜0.05）.Compared with PTs the protein expression of Cx43 had no statistical difference in NLNs（P＞0.05），but significantly increased in MLNs（P＜0.05）.

Conclusions

The interaction of miR-206 and Cx43 probably play a role in the metastasis of axillary lymph node in breast cancer.

Key words: breast neoplasms, metastasis, lymph nodes, microRNA-206, connexin 43

Jia MING, Lin-jun FAN, Ling ZHONG, Qing-qiu CHEN, Jun JIANG. Expressions of miR-206 and Cx43 in primary breast tumours and metastatic lymph nodes[J]. Chinese Journal of Breast Disease(Electronic Edition), 2011, 05(01): 38-41.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhrxbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0807.2011.01.012

https://zhrxbzz.cma-cmc.com.cn/EN/Y2011/V05/I01/38

Figures/Tables 2

基因	上游引物	下游引物
miR-206	5'-UGGAAUGUAAGGAAGUGUGUGG-3'	5'-ACACACUUCCUUACAUUCCAUU-3'
Cx43	5'-GGCGTGACTTCACTACTTTTAAGCA-3'	5'-CAGTTGAGTAGGCTTGAACCTTGTC-3'
GAPDH	5'-ATGTTCGTCATGGGTGTGAAC-3'	5'-CCAAAGTTGTCATGGATGACC-3'

组织	miR-206		Cx43
组织	Ct值（ $\overset{ˉ}{x}$ ±s）	2^-ΔΔCt值	Ct值（ $\overset{ˉ}{x}$ ±s）	2^-ΔΔCt值
N	25.02±0.96	1.000	26.70±0.83	1.000
PTs	18.10±0.62^a	0.021	21.70±0.78^a	0.028
NLNs	18.30±0.65^a	0.024	21.27±2.03^a	0.026
MLNs	17.29±0.37^abc	0.011	20.51±0.65^a	0.023

组织	miR-206		Cx43
组织	Ct值（ $\overset{ˉ}{x}$ ±s）	2^-ΔΔCt值	Ct值（ $\overset{ˉ}{x}$ ±s）	2^-ΔΔCt值
N	25.02±0.96	1.000	26.70±0.83	1.000
PTs	18.10±0.62^a	0.021	21.70±0.78^a	0.028
NLNs	18.30±0.65^a	0.024	21.27±2.03^a	0.026
MLNs	17.29±0.37^abc	0.011	20.51±0.65^a	0.023

References 20

［1］	邵志敏.乳腺癌转移机制的研究及其临床应用［J/CD］.中华乳腺病杂志：电子版，2009，3（5）：475-479.
［2］	Lewis BP，Shih IH，Jones Rhoades MW，et al.Prediction of mammalian microRNA targets［J］.Cell，2003，115（7）：787-798.
［3］	Zhang H，Li Y，Lai M.The microRNA network and tumor metas-tasis［J］.Oncogene，2010，18，29（7）：937-948.
［4］	Tavazoie SF，Alarcón C，Oskarsson T，et al.Endogenous human microRNAs that suppress breast cancer metastasis［J］.Nature，2008，451（7175）：147-152.
［5］	Conklin CM，Bechberger JF，MacFabe D，et al.Genistein and quercetin increase connexin43 and suppress growth of breast cancer cells［J］.Carcinogenesis，2007，28（1）：93-100.
［6］	Elzarrad MK，Haroon A，Willecke K，Dobrowolski R，Gillespie MN，et al.Connexin-43 up-regulation in micrometastases and tumor vasculature and its role in tumor cell attachment to pulmonary endothelium［J］.BMC Med，2008，22（6）：20.
［7］	Kanczuga Koda L，Sulkowski S，Lenczewski A，et al.Increased expression of connexins 26 and 43 in lymph node metastases of breast cancer［J］.J Clin Pathol，2006，59（4）：429-33.
［8］	Inose H，Ochi H，Kimura A，et al.microRNA regulatory mechanism of osteoblast differentiation［J］.Proc Natl Acad Sci USA，2009，106（49）：20794-20799.
［9］	Joseph S，David WR.Molecular Cloning：a laboratory manual［M］.3rd ed.Beijing：Beijing Science Publishing Company，2002：1564-1595.
［10］	He L，Hannon GJ.MicroRNAs：small RNAs with a big role in gene regulation［J］.Nat Rev Genet，2004，5（7）：522-531.
［11］	Hutvagner G.MicroRNAs and cancer：issue summary［J］.Oncogene，2006，25：6154-6155.
［12］	Esquela Kerscher A，Slack FJ.Oncomirs-microRNAs with a role in cancer［J］.Nat Rev Cancer，2006，6（4）：259-269.
［13］	Yu Z，Baserga R，Chen L，Wang C，Lisanti MP，Pestell RG.microRNA，cell cycle，and human breast cancer［J］.Am J Pathol，2010，176（3）：1058-1064.
［14］	Xu X，Francis R，Wei CJ，et al.Connexin 43-mediated modulation of polarized cell movement and the directional migration of cardiac neural crest cells［J］.Development，2006，133（18）：3629-3639.
［15］	Homkajom B，Sims NR，Muyderman H.Connexin 43 regulates astrocytic migration and proliferation in response to injury［J］.Neurosci Lett，2010，486（3）：197-201.
［16］	Kanczuga Koda L，Sulkowski S，Tomaszewski J，et al.Connexins 26 and 43 correlate with Bak，but not with Bcl-2 protein in breast cancer［J］.Oncol Rep，2005，14（2）：325-329.
［17］	Parthasarathi K，Ichimura H，Monma E，et al.Connexin 43 mediates spread of Ca²⁺dependent proinflammatory responses in lung capillaries［J］.J Clin Invest，2006，116（8）：2193-2200.
［18］	Goldberg GS，Moreno AP，Lampe PD.Gap junctions between cells expressing connexin43 or 32 show inverse permselectivity to adenosine and ATP［J］.J Biol Chem，2002，277（39）：36725-36730.
［19］	Oktem G，Bilir A，Ayla S，et al.Role of intercellular communications in breast cancer multicellular tumor spheroids after chemotherapy［J］.Oncol Res，2006，16（5）：225-233.
［20］	Kanczuga Koda L，Sulkowski S，Lenczewski A，et al.Increased expression of connexins 26 and 43 in lymph node metastases of breast cancer［J］.J Clin Pathol，2006，59（4）：429-433.

[1]	Jiajia Wang, Yunyun Zhan, Fan Jiang, Biyun Sun, Yu Bi, Rubing Li, Mei Peng. Application of Peyton's four-step teaching method in standardized residency training program for ultrasound segmentation of cervical lymph nodes [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2024, 21(08): 814-818.
[2]	Yang Li, Jinyu Cai, Xiaozhi Dang, Wanying Chang, Yan Ju, Yi Gao, Hongping Song. Application value of a deep learning-based model for generating strain elastography images using breast grayscale ultrasound images [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2024, 21(06): 563-570.
[3]	Collaboration Group，Breast Cancer Specialty Committee，Hebei Anti-Cancer Association Nursing. Expert consensus on nursing management of central venous access in breast cancer patients [J]. Chinese Journal of Breast Disease(Electronic Edition), 2024, 18(06): 321-329.
[4]	Chenlu Liu, Jie Liu, Fan Zhang, Caiying Yan, Qian Chen, Shuangqing Chen. Habitat analysis based on enhanced MRI radiomic features in predicting HER-2 expression in breast cancer [J]. Chinese Journal of Breast Disease(Electronic Edition), 2024, 18(06): 339-345.
[5]	Xiaoyu Zhang, Yulai Yin, Yinxu Zhang. Efficacy and prognostic analysis of apatinib combined with neoadjuvant chemotherapy in triple negative breast cancer [J]. Chinese Journal of Breast Disease(Electronic Edition), 2024, 18(06): 346-352.
[6]	Lin Qiu, Jinhui Liu, Tuerhong Zumureti, Yuexin Ma, Xiaoling Leng. Diagnostic value of ultrasound radiomics in non-mass breast cancer in dense breasts [J]. Chinese Journal of Breast Disease(Electronic Edition), 2024, 18(06): 353-360.
[7]	Mengmeng Han, Xueyuan Feng, Ning Ma. A case of radial nerve injury after modified radical surgery for breast cancer [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2025, 19(01): 117-118.
[8]	Shizhen Zhou, Xingya Zhu, Qinggang Yuan, Lixiang Liu, Kai Wang, Ji Miao, Chao Ding, Hao Wang, Wenxian Guan. Effect analysis of indocyanine green fluorescence imaging technique in laparoscopic lateral lymph node dissection of rectal cancer [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2025, 19(01): 44-47.
[9]	Jiehong Gao, Pingping Li, Jing Qi, Yinhai Dai. Study on the relationship between the expression of ETFA gene and clinicopathological parameters and prognosis in breast cancer [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2025, 19(01): 64-67.
[10]	Zhizhao Zhang, Rui Wang, Pingping Gao, Chengfang Wang, Cheng Wang, Xiaowei Qi. Relationship between DNMT3B and prognosis of breast cancer and its biological mechanism [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(06): 624-629.
[11]	Lingyan Wang, Chunhui Gao, Xueyuan Feng, Xinmiao Cui, Huan Liu, Wenming Zhao, Jinku Zhang. Application of circulating tumor cells in neoadjuvant and postoperative adjuvant therapy for breast cancer [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(06): 630-633.
[12]	Yi Lin, Wenlong Zhong, Kaiwen Li, Wang He, Tianxin Lin. Multidisciplinary consultation on difficult cases in Guangdong Urological Association （Phase 15）: comprehensive treatment of metastatic bladder cancer [J]. Chinese Journal of Endourology(Electronic Edition), 2024, 18(06): 648-652.
[13]	Jingming Lu, Dawei Han, Yaoxing Ren, Tianxiao Huang, Junxi Xiang, Xufeng Zhang, Yi Lyu, Fumin Wang. Meta-analysis of lymph node metastasis prediction of intrahepatic cholangiocarcinoma based on preoperative radiomics [J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2024, 13(06): 852-858.
[14]	Runjin Zhang, Pan Yang, Yansi Lin, Zunlong Liu, Jianping Liu, Xiaoyan Jin. Epstein-Barr virus-associated cholangiocarcinoma complicated with multiple metastases: a case report and Chinese literature review [J]. Chinese Journal of Hepatic Surgery(Electronic Edition), 2024, 13(06): 865-869.
[15]	Yu Liu, Shaobin Duan, Zhixiang Ding, Zhitao Shi. Expression of miR-34a-5p in colon cancer patients and its correlation with clinical characteristics and prognosis [J]. Chinese Journal of Digestion and Medical Imageology(Electronic Edition), 2024, 14(06): 485-490.

Please choose a citation manager

Content to export