Effect of artesunate on proliferation and morphology of MCF-7 breast cancer cell lines

doi:10.3877/cma.j.issn.1674-0807.2011.01.011

Abstract

Abstract:

Objective

To study the effect of artesunate（ART）on proliferation and morphology of breast cancer MCF-7 cells.

Methods

After MCF-7 cells were treated with ART，MTT-assay was performed to examine the proliferation of MCF-7 cells and observe their morphological changes，and FCM（flow cytometry）assay was used to analyze the distributions of cell cycles and cell apoptosis.Statistical analysis was done using repeated measures analysis of variance.

Results

ART had suppressive effect on MCF-cells and with increase of the concentration and time，the suppressive effect was enhanced（P＜0.05），showing dose-and timedependent.ART could inhibit the proliferation of MCF-7 cells，change the morphological structure of MCF-7 cells，and arrest MCF-7 cells in the S and G2/M phases of cell cycle.ART of 6 μmol/L and 8 μmol/L could cause 3.15%and 8.43%apoptosis of MCF-7 cells，respectively.

Conclusions

ART has the capability of changing morphological structure，inhibiting proliferation of MCF-7 cells and arresting MCF-7 cells in S and G2/M phases in cell cycle.It also induces apoptosis of MCF-7 cells.

Key words: artesunate, breast neoplasms, cell cycle, apoptosis

Xiao-bo ZHAO, Tian-yong XING, Kai-nan WU. Effect of artesunate on proliferation and morphology of MCF-7 breast cancer cell lines[J]. Chinese Journal of Breast Disease(Electronic Edition), 2011, 05(01): 34-37.

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URL: https://zhrxbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0807.2011.01.011

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Figures/Tables 2

References 10

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组别	24 h		48 h		72 h
组别	［D（600）］值	抑制率（%）	［D（600）］值	抑制率（%）	［D（600）］值	抑制率（%）
细胞对照组	0.283±0.027	0.00	0.602±0.017	0.00	0.738±0.008	0.00
NaHCO₃组	0.279±0.004	0.00	0.594±0.022	0.00	0.741±0.012	0.00
2 μmol/L	0.275±0.015	2.83	0.544±0.012	9.63	0.684±0.127	7.32
4 μmol/L	0.268±0.037	5.30	0.478±0.104	25.10	0.573±0.102^a	22.36
6 μmol/L	0.254±0.012	10.24	0.424±0.024^ab	29.57	0.403±0.032^ab	45.39
8 μmol/L	0.242±0.021	14.49	0.401±0.080^ab	33.39	0.352±0.021^abc	52.30
10 μmol/L	0.219±0.018^ab	22.62	0.354±0.015^abc	41.20	0.257±0.014^abc	65.18
20 μmol/L	0.212±0.011^ab	25.09	0.242±0.013^abc	59.80	0.196±0.073^abc	73.44

组别	24 h		48 h		72 h
组别	［D（600）］值	抑制率（%）	［D（600）］值	抑制率（%）	［D（600）］值	抑制率（%）
细胞对照组	0.283±0.027	0.00	0.602±0.017	0.00	0.738±0.008	0.00
NaHCO₃组	0.279±0.004	0.00	0.594±0.022	0.00	0.741±0.012	0.00
2 μmol/L	0.275±0.015	2.83	0.544±0.012	9.63	0.684±0.127	7.32
4 μmol/L	0.268±0.037	5.30	0.478±0.104	25.10	0.573±0.102^a	22.36
6 μmol/L	0.254±0.012	10.24	0.424±0.024^ab	29.57	0.403±0.032^ab	45.39
8 μmol/L	0.242±0.021	14.49	0.401±0.080^ab	33.39	0.352±0.021^abc	52.30
10 μmol/L	0.219±0.018^ab	22.62	0.354±0.015^abc	41.20	0.257±0.014^abc	65.18
20 μmol/L	0.212±0.011^ab	25.09	0.242±0.013^abc	59.80	0.196±0.073^abc	73.44

组别	凋亡率	G0/G1	S	G2/M
细胞对照组	0.00	66.61	19.87	13.52
2 μmol/L	0.00	51.35	16.34	32.31
6 μmol/L	3.15	39.33	36.75	23.92
8 μmol/L	8.43	39.84	42.08	18.08

组别	凋亡率	G0/G1	S	G2/M
细胞对照组	0.00	66.61	19.87	13.52
2 μmol/L	0.00	51.35	16.34	32.31
6 μmol/L	3.15	39.33	36.75	23.92
8 μmol/L	8.43	39.84	42.08	18.08

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