Evaluation of CDK5RAP2 expression in breast cancer by preparing its monoclonal antibody

doi:10.3877/cma.j.issn.1674-0807.2008.05.008

Abstract

Abstract:

Objective

To evaluate the difference of CDK5RAP2 expression between normal breast tissue(N) and malignant breast cancer by its monoclonal antibody.

Methods

Monoclonal antibody of CDK5RAP2 was prepared by means of hybridoma technique and its specificity was tested by immunofluorescence on the cell knockout CDK5RAP2. Three groups of samples: normal breast tissue, ductal carcinoma in situ (DCIS) and infiltrating ductal carcinoma (IDC) (30 cases in each group) were selected.Immunohistochemistry(IHC) was performed on paraffin blocks by the CDK5RAP2 McAb to determine the expression patterns of CDK5RAP2 in breast cancer and normal breast tissue respectively.

Results

One antibody against CDK5RAP2 (5F6) with IgG1 subtype was obtained. IHC staining indicated that the CDK5RAP2 expression in both IDC and DCIS cases was higher than that in normal group(P ＜0.001), but there was no difference between IDC and DCIS groups.

Conclusion

CDK5RAP2 is not expressed or lowly expressed in normal breast tissue. The CDK5RAP2 expression is increasingly enhanced in breast cancer tissues to lead to breast cancer occurrence and progressing. Therefore CDK5RAP2 may be a potential indicator of breast cancer.

Key words: CDK5RAP2, Breast cancer, Monoclonal antibody

Lin ZHANG, Tong-wen ZHANG, Yi YANG, Meng-hui LI, Yi LUO, Xi-yin WEI, Yun NIU, Ri-fang NIU, Zhong QI. Evaluation of CDK5RAP2 expression in breast cancer by preparing its monoclonal antibody[J]. Chinese Journal of Breast Disease(Electronic Edition), 2008, 02(05): 31-34.

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