切换至 "中华医学电子期刊资源库"

中华乳腺病杂志(电子版) ›› 2011, Vol. 05 ›› Issue (06) : 660 -669. doi: 10.3877/cma.j.issn.1674-0807.2011.06.002

临床研究

乳腺球蛋白在术前乳腺癌和腋窝淋巴结细针穿刺吸取组织中的表达及临床意义
方先勇1, 朱立强1,(), 江涛1, 朱玉秋1   
  1. 1.221002 江苏徐州,徐州医学院附属医院细胞室
  • 收稿日期:2011-04-08 出版日期:2011-12-01
  • 通信作者: 朱立强
  • 基金资助:
    徐州市科技计划项目(XM08C089)

Expression of mammaglobin in preoperatively aspirated breast cancer and axillary lymph node tissues and clinical significance

Xian-yong FANG1, Li-qiang ZHU1,(), Tao JIANG1, Yu-qiu ZHU1   

  1. 1.Clinic Laboratory of the Affiliated Hospital of Xuzhou Medical College,221002 Xuzhou, China
  • Received:2011-04-08 Published:2011-12-01
  • Corresponding author: Li-qiang ZHU
引用本文:

方先勇, 朱立强, 江涛, 朱玉秋. 乳腺球蛋白在术前乳腺癌和腋窝淋巴结细针穿刺吸取组织中的表达及临床意义[J/OL]. 中华乳腺病杂志(电子版), 2011, 05(06): 660-669.

Xian-yong FANG, Li-qiang ZHU, Tao JIANG, Yu-qiu ZHU. Expression of mammaglobin in preoperatively aspirated breast cancer and axillary lymph node tissues and clinical significance[J/OL]. Chinese Journal of Breast Disease(Electronic Edition), 2011, 05(06): 660-669.

目的

探讨乳腺球蛋白(mammaglobin, MAM)在术前乳腺癌及腋窝淋巴结细针穿刺吸取组织中的表达及临床意义。

方法

对91 例原发性乳腺癌行术前细针穿刺,将吸取的组织制备涂片,采用免疫组织化学方法(EnVision 法)检测MAM 表达,观察MAM 表达与临床病理指标的关系。 同时另收集15 例原发性肺癌腋窝淋巴结转移患者,分析乳腺癌腋窝淋巴结转移、乳腺癌增生性淋巴结炎和肺癌腋窝淋巴结转移三者间术前腋窝淋巴结MAM 表达的区别。 统计分析采用χ2 检验。

结果

在91 例原发性乳腺癌患者中MAM 的阳性表达率为74.72%(68/91)。 MAM 的阳性表达与雌激素受体(ER)、孕激素受体(PR)、组织学分级、细胞学分级有关(P<0.05),与患者的年龄、肿瘤大小、淋巴结是否转移及HER-2 状态无关(P>0.05)。 腋窝淋巴结观察结果显示,MAM在乳腺癌腋窝淋巴结转移患者中呈阳性表达,在乳腺癌增生性腋窝淋巴结炎和肺癌腋窝淋巴结转移患者中均为阴性表达。

结论

MAM 可表达于原发性和转移性乳腺癌,细针穿刺细胞学检查结合MAM 的测定对乳腺癌的诊断及鉴别具有一定辅助判断价值。MAM 阳性表达与ER、PR 状态、细胞学分级、组织学分级有关,有利于了解肿瘤的生物学指标及判断预后。

Objective

To investigate the expression of mammaglobin (MAM) in preoperative breast carcinoma and axillary lymph node tissues using fine-needle aspiration(FNA) biopsy and its clinical significance.

Methods

Ninety-one cases of primary breast carcinoma were enrolled into the study. All cases received preoperative fine-needle aspiration biopsy. Immunocytochemistry for testing MAM was performed in all the cases using EnVision method to observe the relation between MAM positive expression and cytologic grading and clinicopathological parameters. In the meantime, 15 primary lung cancer patients were collected, with an intention of comparing the MAM expression in metastatic axillary lymph node of breast cancer, hyperplastic axillary lymphadenitis of breast cancer and metastatic axillary lymph node of primary lung cancer. Statistical analysis was performed using chi-square test.

Results

The positive rate of MAM in primary breast carcinoma patients was 74.72% (68/91). The positive expression of MAM was associated with estrogen receptor(ER), progesterone receptor (PR), cytological grade and histologic grade (P <0. 05),while the MAM expression level was independent of age, tumor size, the status of axillary lymph node metastasis and HER-2 (P >0. 05). The observation of MAM expression in axillary lymph nodes showed MAM positively expressed in the metastatic axillary lymph node of breast cancer, but negatively expressed in the hyperplastic axillary lymphadenitis of breast cancer and the metastatic axillary lymph node of primary lung cancer.

Conclusion

MAM expresses in both primary and metastatic breast carcinomas. FNA combined with testing MAM may be helpful for diagnosis and identification of breast cancer. The correlation of MAM positive expression with the status of ER, PR, cytological grade and histologic grade may have a potential value for preoperatively assessing the biological characteristics and the prognosis of the patients.

表1 免疫细胞化学染色阳性程度的判定系统
表2 乳腺癌细胞学分级的评分标准
图1 病理图片(EnVision 法 ×400) 浸润性导管癌细针穿刺吸取细胞涂片乳腺球蛋白(>10%的肿瘤细胞)呈阳性表达。
表3 乳腺球蛋白(MAM)表达与临床病理因素的关系
图2 病理图片(EnVision 法 ×400) 浸润性导管癌腋窝淋巴结转移,腋窝淋巴结细针穿刺吸取细胞涂片乳腺球蛋白( >10%的转移性癌细胞)呈阳性表达。
图3 病理图片(EnVision 法 ×400) 原发性肺癌腋窝淋巴结转移,腋窝淋巴结细针穿刺吸取细胞涂片乳腺球蛋白呈阴性表达。
图4 病理图片(EnVision 法 ×400) 乳腺癌增生性淋巴结炎,腋窝淋巴结细针穿刺吸取细胞涂片乳腺球蛋白呈阴性表达。
[1]
Watson MA, Dintzis S, Darrow CM, et al. Mammaglobin expression in primary,metastatic, and occult breast cancer [J].Cancer Res,1999,59 (13):3028-3031.
[2]
Tanaka K, Shoji T, Tominaga Y, et al. Statistical analysis of diagnostic failure of fine needle aspiration cytology (FNAC)in breast cancer [J]. J Surg Oncol,2001,76(2):100-105.
[3]
Rita N, Cecilia B, Pellegrino C,et al. Immunocytochemical evaluation of HER-2/neu on fine-needle aspirates from primary breast carcinomas [J]. Diagn Cytopathol,2003,28(3):142-146.
[4]
Robinsin IA, McKee G, Nicholson A, et al. Prognostic value of cytological grading of fine-needle aspirates from breast carcinomas [J]. Lancet,1994,343(8903):947-949.
[5]
Taniguchi E, Yang Q, Tang W, et al. Cytologic grading of invasive breast carcinoma: correlation with clinicopathologic variables and predictive value of nodal metastasis [J]. Acta Cytol,2000,44(4):587 -591.
[6]
《乳腺癌HER2 检测指南》编写组.乳腺癌HER2 检测指南[J].中华病理学杂志,2006,35(10):631-633.
[7]
Watson MA, Fleming TP. Mammaglobin, a mammary specific member of the uteroglobin gene family is overexpressed in human breast cancer [J]. Cancer Res,1996,56 (4):860-865.
[8]
O'Brien N,Maguire TM, O'Donovan N, et al. Mammaglobin A: a promising marker for breast cancer [J]. Clin Chem,2002,48 ( 8):1362-1364.
[9]
Fritzsche FR, ThomasA,Winzer KJ, et al. Co-expression and prognostic value of gross cystic disease fluid protein 15 and mammaglobin in primary breast cancer [J]. Histol Histopathol,2007,22(11):1221-1230.
[10]
Ariga R, Bloom K, Reddy VB, et al. Fine-needle aspiration of clinically suspicious palpable breast masses with histopathologic correlation [J]. Am J Surg,2002,184(5):410-413.
[11]
Sneige N. Utility of cytologic specimens in the evaluation of prognostic and predictive factors of breast cancer: current issues and future directions [J]. Diagn Cytopathol,2004,30(3):158-165.
[12]
Perou CM, Sorlie T, Eisen MB, et al. Molecular portraits of human breast tumors [J]. Nature,2000,406(17):747-752.
[13]
Liu H, Fan Q, Zhang Z, et al. Basal-HER-2 phenotype shows poorer survival than basal-like phenotype in hormone receptor-negative invasive breast cancers [J]. Hum Pathol,2008,39(2):167 -174.
[14]
Guan XF, Hamedani MK, Adeyinka A, et al. Relationship between mammaglobin expression and estrogen receptor status in breast tumors [J]. Endocrine,2003,21(3):245-250.
[15]
Pan PN, Waanders E, Manders P, et al. Mammaglobin is associated with low-grade, steroid receptor positive breast tumors from postmenopausal patients, and has independent prognostic value for relapse-free survival time [J]. J Clin Oncol,2004,22 (4):691-698.
[16]
Houghton RL, DillonDC, Molesh DA, et al. Transcriptional complementarity in breast cancer: application to detection of circulating tumor cells [J]. Mol Diagn,2001,6 (2):79 -91.
[17]
Zafrakas M, Petschke B, Donner A, et al. Expression analysis of mammaglobin A (SCGB2A2) and lipophilin B(SCGB1D2) in more than 300 human tumors and matching normal tissues reveals their co-expression in gynecologic malignancies [J]. BMC Cancer,2006,6:88.
[18]
Sjodin A, Guo D, Hofer PA, et al. Mammaglobin in normal human sweat glands and human sweat gland tumors [J]. J Invest Dermatol,2003,121 (2):428 -429.
[1] 李洋, 蔡金玉, 党晓智, 常婉英, 巨艳, 高毅, 宋宏萍. 基于深度学习的乳腺超声应变弹性图像生成模型的应用研究[J/OL]. 中华医学超声杂志(电子版), 2024, 21(06): 563-570.
[2] 河北省抗癌协会乳腺癌专业委员会护理协作组. 乳腺癌中心静脉通路护理管理专家共识[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(06): 321-329.
[3] 刘晨鹭, 刘洁, 张帆, 严彩英, 陈倩, 陈双庆. 增强MRI 影像组学特征生境分析在预测乳腺癌HER-2 表达状态中的应用[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(06): 339-345.
[4] 张晓宇, 殷雨来, 张银旭. 阿帕替尼联合新辅助化疗对三阴性乳腺癌的疗效及预后分析[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(06): 346-352.
[5] 邱琳, 刘锦辉, 组木热提·吐尔洪, 马悦心, 冷晓玲. 超声影像组学对致密型乳腺背景中非肿块型乳腺癌的诊断价值[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(06): 353-360.
[6] 韩萌萌, 冯雪园, 马宁. 乳腺癌改良根治术后桡神经损伤1例[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 117-118.
[7] 周世振, 朱兴亚, 袁庆港, 刘理想, 王凯, 缪骥, 丁超, 汪灏, 管文贤. 吲哚菁绿荧光成像技术在腹腔镜直肠癌侧方淋巴结清扫中的应用效果分析[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 44-47.
[8] 高杰红, 黎平平, 齐婧, 代引海. ETFA和CD34在乳腺癌中的表达及与临床病理参数和预后的关系研究[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 64-67.
[9] 贺斌, 马晋峰. 胃癌脾门淋巴结转移危险因素[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 694-699.
[10] 张志兆, 王睿, 郜苹苹, 王成方, 王成, 齐晓伟. DNMT3B与乳腺癌预后的关系及其生物学机制[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 624-629.
[11] 王玲艳, 高春晖, 冯雪园, 崔鑫淼, 刘欢, 赵文明, 张金库. 循环肿瘤细胞在乳腺癌新辅助及术后辅助治疗中的应用[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 630-633.
[12] 赵林娟, 吕婕, 王文胜, 马德茂, 侯涛. 超声引导下染色剂标记切缘的梭柱型和圆柱型保乳区段切除术的效果研究[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 634-637.
[13] 林逸, 钟文龙, 李锴文, 何旺, 林天歆. 广东省医学会泌尿外科疑难病例多学科会诊(第15期)——转移性膀胱癌的综合治疗[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(06): 648-652.
[14] 刘郁, 段绍斌, 丁志翔, 史志涛. miR-34a-5p 在结肠癌患者的表达及其与临床特征及预后的相关性研究[J/OL]. 中华消化病与影像杂志(电子版), 2024, 14(06): 485-490.
[15] 王曦娅, 尹弘青, 丁伟, 徐滨, 于海源, 马东升, 邵军. 桥本背景下甲状腺乳头状癌多参数分析预测大容量淋巴结转移[J/OL]. 中华临床医师杂志(电子版), 2024, 18(06): 548-554.
阅读次数
全文


摘要