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中华乳腺病杂志(电子版) ›› 2011, Vol. 05 ›› Issue (05) : 542 -549. doi: 10.3877/cma.j.issn.1674-0807.2011.05.005

临床研究

金属蛋白酶组织抑制因子1 在乳腺癌组织中的表达及其与多种生物学指标的关系
张绍坤1,(), 郭冰2, 王雷1, 宁均1, 周玉峰1, 李春来1   
  1. 1.157011 黑龙江 牡丹江,牡丹江市第一人民医院乳腺科
    2.266033 青岛,青岛市海慈医院
  • 收稿日期:2010-06-25 出版日期:2011-10-01
  • 通信作者: 张绍坤
  • 基金资助:
    黑龙江省牡丹江市科技项目(G2010S0011)

Expression of tissue inhibitor of metalloproteinase-1 in breast cancer tissue and its relationship with multiple biomarkers

Shao-kun ZHANG,1(), Bing GUO1, Lei WANG1, Jun NING1, Yu-feng ZHOU1, Chun-lai LI1   

  1. 1.Department of Breast Surgery, First People's Hospital of Mudanjiang City, Mudanjiang 157011, China
  • Received:2010-06-25 Published:2011-10-01
  • Corresponding author: Shao-kun ZHANG
引用本文:

张绍坤, 郭冰, 王雷, 宁均, 周玉峰, 李春来. 金属蛋白酶组织抑制因子1 在乳腺癌组织中的表达及其与多种生物学指标的关系[J/OL]. 中华乳腺病杂志(电子版), 2011, 05(05): 542-549.

Shao-kun ZHANG, Bing GUO, Lei WANG, Jun NING, Yu-feng ZHOU, Chun-lai LI. Expression of tissue inhibitor of metalloproteinase-1 in breast cancer tissue and its relationship with multiple biomarkers[J/OL]. Chinese Journal of Breast Disease(Electronic Edition), 2011, 05(05): 542-549.

目的

研究乳腺癌组织中金属蛋白酶组织抑制因子1(TIMP-1)的表达及其与增殖细胞核抗原ki67、P53 癌基因(P53 gene)、 人表皮生长因子受体-2 (HER-2)表达的关系。

方法

应用免疫组织化学方法(SP 法)检测104 例乳腺癌和33 例癌旁乳腺组织TIMP-1 的表达情况以及乳腺癌组织Ki67、P53、HER-2 的表达情况。非等级计数资料比较采用χ2 检验,等级计数资料比较采用Mann-Whitney U 或Kruskal-Wallis H非参数秩和检验。两指标间的相关性采用Spearman 相关分析。

结果

TIMP-1 在乳腺癌组织中的阳性表达率为69.23%(72/104),在癌旁乳腺组织中的阳性表达率为15.15%(5/33),二者间差异有统计学意义(χ2=29.76,P=0.00)。TIMP-1 表达与年龄、月经状态、手术方式、临床分期、肿瘤病理类型等因素无关(P>0.05) , 与肿瘤直径、组织学分级、淋巴结转移有关(P<0.01)。TIMP-1 阳性表达与Ki67 表达呈正相关(r=0.28,P<0.01),与P53 表达呈负相关(r=-0.42,P<0.01),与HER-2 表达不相关(r=-0.01,P>0.05)。

结论

TIMP-1 在乳腺癌组织中表达增高,且与乳腺癌侵袭过程关系密切;TIMP-1 与多种生物学指标联合检测能更准确地预测预后、指导治疗。

Objective

To investigate the expression of tissue inhibitor of metalloproteinase-1 in breast cancer tissue and its relationship with Ki67, P53 and HER-2.

Methods

The immunohistochemistry method was used to detect the expressions of TIMP-1,Ki67,P53 and HER-2 in the tissues of 104 breast cancer specimens and 33 pericancer specimens.χ2 test was used to analyze the unranked qualitative data.Mann-Whitney U test or Kruskal-Wallis H test was used for comparison between ranked qualitative data.Spearman rank correlation test was conducted for analysis of correlation of TIMP-1 with Ki67, P53 and HER-2 in breast cancer.

Results

The positive expression rate of TIMP-1 was 69.23%(72/104)in cancer tissue, and 15.15%(5/33)in pericancer tissue, with significant difference between them(χ2=29.76, P=0.00).The positive expression of TIMP-1 was not related with age, menstrual status, operation modes, clinical stage, and pathological type of cancer(P>0.05), but correlated with tumor size, histological grades and lymph node metastasis(P<0.01).The positive expression of TIMP-1 was positively correlated with Ki67(r=0.28,P<0.01), negatively correlated with p53(r=-0.42,P<0.01), and not correlated with HER-2(r=-0.01,P>0.05).

Conclusion

The high expression of TIMP-1 in breast cancer tissue is highly correlated with invasion.Detection of TIMP-1 combined with other biomarkers can accurately indicate the outcome and instruct treatment for breast cancer.

表1 乳腺癌组织和癌旁组织中TIMP-1 的表达
图1 乳腺癌组织中TIMP-1 阳性(DAB 染色 ×400)
图2 乳腺癌组织中TIMP-1 阴性 (DAB 染色 ×100)
表2 金属蛋白酶组织抑制因子1 表达与临床病理因素的关系
表3 乳腺癌组织中金属蛋白酶组织抑制因子1 与Ki67、P53、HER-2 表达的相关性
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