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中华乳腺病杂志(电子版) ›› 2008, Vol. 02 ›› Issue (02) : 22 -24. doi: 10.3877/cma.j.issn.1674-0807.2008.02.006

实验研究

TNFR II 基因-196M /R 多态性与乳腺癌易感性的相关性
柳晓义1, 胡建霞1, 王宇1, 曹明智1,()   
  1. 1.266003 青岛,青岛大学医学院附属医院乳腺外科
  • 收稿日期:2008-01-10 出版日期:2008-04-01
  • 通信作者: 曹明智

Association between TNFR II gene polymorphisms and breast cancer

Xiao-yi LIU1, Jian-xia HU1, Yu WANG1, Mingzhi CAO,1()   

  1. 1.Department of Galactophore, Qingdao Medical School Hospital, Qingdao 266003, China
  • Received:2008-01-10 Published:2008-04-01
  • Corresponding author: Mingzhi CAO
引用本文:

柳晓义, 胡建霞, 王宇, 曹明智. TNFR II 基因-196M /R 多态性与乳腺癌易感性的相关性[J/OL]. 中华乳腺病杂志(电子版), 2008, 02(02): 22-24.

Xiao-yi LIU, Jian-xia HU, Yu WANG, Mingzhi CAO. Association between TNFR II gene polymorphisms and breast cancer[J/OL]. Chinese Journal of Breast Disease(Electronic Edition), 2008, 02(02): 22-24.

目的

探讨TNF-α 受体II(TNFR II)基因第6 外显子-196M/R 多态性与中国北方女性乳腺癌发病的相关性。

方法

采用聚合酶链反应-限制性片段长度多态性( PCR-RFLP)方法检测TNFR II 基因-196M/R 多态性,分析212 例乳腺癌患者(实验组),218 例乳腺良性病变患者(良性组)和220 例健康对照者(对照组)的TNF-α 受体II(TNFR II)基因多态性与乳腺癌易感性的关系。

结果

(1)650 例研究对象中存在TNFR II 基因-196M/R 多态性。 (2) -196M/R 位点的TG+GG 基因型及G 等位基因频率在良性病变组与对照组间差异无统计学意义,而在乳腺癌组则显著高于良性病变组和对照组(均P <0.05)。

结论

TNFR II 基因-196M/R 在乳腺癌组和对照组存在多态性,第6 外显子-196M/R位点T/G 多态性可能为乳腺癌发病的易感基因位点,G 等位基因可能为中国北方女性乳腺癌发病的易感基因。

Objective

To explore the relationship between TNF-α receptor II(TNFR II)gene -196M/R polymorphisms and breast cancer.

Methods

TNFR II gene polymorphisms at position -196 were determined by polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) method in 212 female patients with breast cancer,218 female patients with breast benign diseases and 220 female healthy controls.

Results

(1)There were TNFR II gene -196 M/R polymorphisms in the 640 cases. (2) There was no statistic difference in the frequencies of TG+GG genotype and allele G between benign and control groups. The frequencies of TG+GG genotype in the study group were significantly higher than those in the benign and control groups (P <0.05).

Conclusion

There were TNFR II gene -196 M/R polymorphisms in breast cancer and control groups.TNFR II gene -196 G allele may be the predisposing gene and the relative risk of breast cancer.

表1 TNFR II 基因第6 外显子-196M/R 位点基因型和等位基因的分布频率
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