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中华乳腺病杂志(电子版) ›› 2012, Vol. 06 ›› Issue (03) : 279 -286. doi: 10.3877/cma. j. issn.1674-0807.2012.03.007

论著

连接蛋白43 和上皮细胞钙黏蛋白在乳腺浸润性导管癌组织中表达的意义
高一菁1, 陈战1, 李锐1, 谢晓梅1,(), 涂永久1,()   
  1. 1.361003 福建省厦门市,解放军第174 医院普通外科
  • 收稿日期:2012-04-05 出版日期:2012-06-01
  • 通信作者: 谢晓梅, 涂永久

Significance of expressions of connexin 43 and E-cadherin in invasive ductal carcinoma

Yi-jing GAO1, Zhan CHEN1, Rui LI1, Xiao-mei XIE,1(), Yong-jiu TU,1()   

  1. 1.Department of General Surgery,The 174th Hospital of PLA,361003 Xiamen,China
  • Received:2012-04-05 Published:2012-06-01
  • Corresponding author: Xiao-mei XIE, Yong-jiu TU
引用本文:

高一菁, 陈战, 李锐, 谢晓梅, 涂永久. 连接蛋白43 和上皮细胞钙黏蛋白在乳腺浸润性导管癌组织中表达的意义[J/OL]. 中华乳腺病杂志(电子版), 2012, 06(03): 279-286.

Yi-jing GAO, Zhan CHEN, Rui LI, Xiao-mei XIE, Yong-jiu TU. Significance of expressions of connexin 43 and E-cadherin in invasive ductal carcinoma[J/OL]. Chinese Journal of Breast Disease(Electronic Edition), 2012, 06(03): 279-286.

目的

探讨间隙连接蛋白43(connexin 43,Cx43)及上皮细胞钙黏蛋白(E-cadherin,E-cad)在乳腺浸润性导管癌组织中表达的作用。

方法

采用Elivision 免疫组织化学法对17 例正常乳腺组织、42 例乳腺良性病变组织(18 例乳腺纤维腺瘤、24 例纤维腺病)及41 例乳腺浸润性导管癌组织中的Cx43 和E-cad 表达进行检测。 统计分析采用卡方检验、配对计数资料的McNemar 检验及Kappa 检验。

结果

Cx43 和E-cad 在浸润性导管癌中表达明显降低,与TNM 分期、病理组织学分级及淋巴结转移有关(P<0.050)。 在浸润性导管癌中Cx43 和E-cad 蛋白表达一致性较好(Kappa 值=0.466,P=0.003)。

结论

Cx43 和E-cad 的表达下降可能在乳腺浸润性导管癌发生和发展过程中发挥一定的作用。 联合检测Cx43 和E-cad 有利于判断肿瘤的分化程度及浸润转移情况。

Objective

To investigate the expressions of connexin 43(Cx43) and E-cadherin(E-cad) in invasive ductal carcinoma (IDC) and their clinical significance.

Method

The expressions of Cx43 and E-cad proteins were detected in 41 cases of breast invasive ductal carcinoma,42 cases of benign breast diseases and 17 cases of normal breast tissues by immunohistochemical Elivision method. Chi-square test, McNemar test and Kappa test were used for statistical analysis.

Results

The expressions of Cx43 and E-cad were remarkably decreased in IDC tissues. Both Cx43 and E-cad expressions were significantly related with TNM stage, histologic grading and lymphatic metastasis in breast IDC(P<0.050).There was better agreement between Cx43 and E-cad expressions (Kappa=0.466,P=0.003).

Conclusion

The decreased expressions of Cx43 and E-cad in IDC might play a role in carcinogenesis and development of IDC. It is conducive to judge tumor differentiation and invasion status by detecting Cx43 and E-cad together.

表1 Cx43 和E-cad 在不同的乳腺组织中表达的比较
图1 良性病变中Cx43 的表达定位于胞质,其中以肌上皮细胞明显,于靠近胞膜处有染色质聚积(EP ×100) a:乳腺纤维瘤;b:乳腺纤维腺病
图2 浸润性导管癌Ⅰ、Ⅱ级Cx43 的表达有所减弱(EP ×100)
图3 浸润性导管癌中Ⅲ级Cx43 的表达明显减弱,少数细胞出现胞核异位表达(EP ×100)
图4 良性病变E-cad 的表达在胞膜处成线性染色(EP ×100) a:乳腺纤维瘤;b:乳腺纤维腺病
图5 浸润性导管癌Ⅰ、Ⅱ级E-cad 的表达有所减弱(EP ×100)
图6 浸润性导管癌Ⅲ级E-cad 的表达明显减弱(EP ×100)
表2 乳腺浸润性导管癌中的Cx43 和E-cad 蛋白的表达与临床病理特征的关系
表3 41 例浸润性导管癌组织中Cx43 与E-cad 蛋白的表达关系
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