Abstract:
Objective
To evaluate the effect of sequential use of 5-aza-2’-deoxycytidine (DAC) and cisplatin(PDD) on the proliferation in vitro, cell cycle and apoptosis of triple-negative breast cancer(TNBC)cell line MDA-MB-231.
Methods
The cells were divided into 4 groups: control group,DAC group(treated with 5 μmol/L DAC),PDD group (treated with 15 μmol/L PDD),DAC→PDD group(treated with 2.5 μmol/L DAC for 24 h and then 8 μmol/L PDD for 48 h). MTT and flow cytometry were used to determine the proliferation rate, cells cycles and cells apoptosis rate of MDA-MB-231 cells. Jin’s formula was used to evaluate the combining effect of DAC and PDD.
Results
The proliferation rate of DAC→PDD group was higher than that of the other two groups(P＜0.01).The q values of DAC→PDD group were 1.12 at 48 h,1.17 at 72 h, which indicated the synergistic effect. In DAC →PDD group, the cells in G1 and S stage were decreased, and G2/M cells were increased.The cell apoptosis rate of DAC→PDD group was higher than that of the other two groups(P＜0.01).
Conclusion
The sequential use of DAC and PDD at a low dosage can inhibit the proliferation and promote the apoptosis of human TNBC cells MDA-MB-231.
Key words:
breast neoplasms,
cell proliferation,
cell cycle,
cell apoptosis,
5-aza-2’-deoxycytidine,
cisplatin
San-di SHEN, Zhuo-rong CHEN, Gao-fang XIAO, Yan-min LIU, Zhan HUANG, Zhi-hui LUO. Effect of sequential treatment of 5-aza-2’-deoxycytidine and cisplatin in MDA-MB-231 cells[J]. Chinese Journal of Breast Disease(Electronic Edition), 2013, 07(03): 188-191.