Abstract:
Objective
To explore the effect of exogenous estrogen receptor-β1(ERβ1)gene on the expression of p21 as well as the changes in proliferation ability of MDA-MB-231 cell line by transfecting recombinant eukaryotic expressing vector containing ERβ1cDNA into human breast cancer cell MDA-MB-231,and to investigate the biological role of Erβ1 in breast cancer.
Methods
Recombinant eukaryotic expressing vector containing ERβ1cDNA was transfected into human breast cancer cell MDA-MB-231 by using cationic liposome Lipofectamine 2000 TM as transfecting agent.The expression levels in both mRNAs and the protein levels of both ERβ1 and p21 were tested by real-time polymerase chain reaction (RT-PCR)and Western blot,respectively.The changes of proliferation ability in MDA-MB-231 was displayed by cell growth curve,and the changes in cell apoptosis were detected by flow cytometry.Statistical analysis was performed using t test and analysis of variance.
Results
The proliferation ability decreased and the expression levels of both ERβ1 and p21 m RNA and protein increased significantly in the transfection group compared with the control group(P＜0.010).The rate of cell apoptosis increased from 1.4%to 6.14%(t=-7.960,P=0.001).
Conclusions
ERβ1 can inhibit the cell growth of MDA-MB-231 cells by upregulating the expression of p21.
Key words:
Breast neoplasms,
Estrogen receptor-β1,
p21
Li CHEN, Xin-hua YANG, Lin REN, Cheng YANG, Lingling ZHANG, Jun JIANG. ERβ1 inhibits the growth of breast cancer MDA-MB-231 cell by upregulating p21 expression[J]. Chinese Journal of Breast Disease(Electronic Edition), 2010, 04(04): 427-433.