Study on CD44+/CD24-/low and CD24+tu mor cells in invasive micropapillary carcinoma of the breast

doi:10.3877/cma.j.issn.1674-0807.2009.05.010

Abstract

Abstract:

Objective

To study the stem cell phenotype of invasive micr opapillary carcino ma(I MPC)of the breast.Through the stem cell and epithelial-mesenchy mal transition,the causes of biological behaviour of high metastasis and invasion of IMPC was investigated.

Methods

The expression,l ocation and distribution of CD44 and CD24 were deter mined by immunohistochemical double staining in 82 cases of IMPC and 80 cases of invasive ductal carcino ma not other wise specified (IDC-NOS),under going without preoperative chemot herapy and radiot herapy, and their relationship with clinicopat hological feat ures was anal yzed.St udent'st test was used for quantitative data.Mann-Whitney Utest,Kruskal-Wallis test andχ² test or revisedχ² test were used for qualitative data.Spear man's test was used for association bet ween groups.

Results

The positive expression rate of CD44⁺/CD24^-/low tumor cells was higher in the IMPC gr oup(48.8%,40/82 cases)t han in the IDC-NOS gr oup(31.3%,25/80 cases)(χ²=5.180,P=0.023).The CD44⁺/CD24^-/low tu mor cells was also detected in adjacent stro ma surrounding the micropapillary structure in 53.7%(44/82 cases)of I MPC,with Vi mentin andα-SMA positive and E-Cadherin negative,but scarce in stro ma of IDC-NOS.(3)The CD44⁺/CD24^-/low tu mor cells in micr opapillar y str ucture of IMPC was positively associated with that instroma(r=0.516,P＜0.001),moreover,they were bot h associated withly mphovascular invasion and extranodal extension respectively,and there was statistical difference between lymphovascular invasion and extranodal extension(P＜0.050),that is the positive expression rate of CD44⁺/CD24^-/l^ow in ly mphovascular invasion and extranodal extension was high.(4)The positive expression rate of CD24⁺t u mor cells was also higher in IMPC (65/82 cases)than in IDC-NOS(48/80 cases)(χ²=7.126,P=0.008),and in IMPC the expression of CD24⁺ was higher in positively mphnode metastasis than in negative,with statistical difference(χ²=8.834,P=0.003).

Conclusions

The existence of stem cells and epithelial-mesenchy mal transition in IMPC tumor cells may play an important rolein aggressiveness and higher metastatic risk of IMPC of the breast.It can be a method to develop drugs targeting breast cancer stemcells.

Key words: Breast neoplasms, IMPC, Stem cells, CD44⁺/CD24^-/low, CD24⁺, Epithelial-mesenchy mal transition

Wei-dong LI, Fang-fang LIU, Xin-sheng XU, Bing-bing LIU, Li-fang CUI, Jia WEI, Xiao-jing GUO, Rong-gang LANG, Yu FAN, Feng GU, Li FU. Study on CD44⁺/CD24^-/low and CD24⁺tu mor cells in invasive micropapillary carcinoma of the breast[J]. Chinese Journal of Breast Disease(Electronic Edition), 2009, 03(05): 524-534.

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References 26

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分组	年龄(岁)	肿瘤大小(cm)	组织学分级(例)			LNM(例)		pTNM分期(例)			ENE(例)		LVI(例)		CD24^-(例)		CD24⁺(例)
分组	年龄(岁)	肿瘤大小(cm)	Ⅰ	Ⅱ	Ⅲ	-	+	Ⅰ	Ⅱ	Ⅲ～Ⅳ	-	+	-	+	-	+	-	+
IMPC	54.79±11.17	3.20±1.46	10	48	24	7	75	4	31	47	30	52	24	58	42	40	17	65
IDC-NOS	51.99±9.95	2.58±1.16	7	59	14	37	43	10	52	18	65	15	69	11	55	25	32	48
统计量	1.686	3.000	1.079			29.112		19.958			33.308		53.772		5.180		7.126
P值	0.094^a	0.003^a	0.299^b			0.000^c		0.000^b			0.000^c		0.000^c		0.023^c		0.008^c

分组	年龄(岁)	肿瘤大小(cm)	组织学分级(例)			LNM(例)		pTNM分期(例)			ENE(例)		LVI(例)		CD24^-(例)		CD24⁺(例)
分组	年龄(岁)	肿瘤大小(cm)	Ⅰ	Ⅱ	Ⅲ	-	+	Ⅰ	Ⅱ	Ⅲ～Ⅳ	-	+	-	+	-	+	-	+
IMPC	54.79±11.17	3.20±1.46	10	48	24	7	75	4	31	47	30	52	24	58	42	40	17	65
IDC-NOS	51.99±9.95	2.58±1.16	7	59	14	37	43	10	52	18	65	15	69	11	55	25	32	48
统计量	1.686	3.000	1.079			29.112		19.958			33.308		53.772		5.180		7.126
P值	0.094^a	0.003^a	0.299^b			0.000^c		0.000^b			0.000^c		0.000^c		0.023^c		0.008^c

间质中CD24^-细胞	微乳头结构中CD24^-细胞(例)		r值	P值
间质中CD24^-细胞	-	+	r值	P值
-	30	8
+	12	32	0.516	0.000

间质中CD24^-细胞	微乳头结构中CD24^-细胞(例)		r值	P值
间质中CD24^-细胞	-	+	r值	P值
-	30	8
+	12	32	0.516	0.000

临床病理学特征	CD44⁺/CD24^-/low				CD24⁺
临床病理学特征	阴性(例)	阳性(例)	统计量	P值	阴性(例)	阳性(例)	统计量	P值
年龄
≤55	18	22			10	30
＞55	24	18	-1.093	0.274^a	7	35	-0.925	0.355^a
肿瘤大小(cm)
≤2	9	7			5	11
2～5	29	31			12	48
＞5	4	2	0.000	1.000^b	0	6	2.402	0.121^b
组织学分级
Ⅰ	7	3			0	10
Ⅱ	23	25			10	38
Ⅲ	12	12	0.471	0.493^b	7	17	2.995	0.083^b
淋巴结转移
-	3	4			5	2
+	39	36	0.005	0.946^c	12	63	8.834	0.003^c
pTNM分期
Ⅰ	2	2			2	2
Ⅱ	14	17			6	25
Ⅲ～Ⅳ	26	21	0.656	0.418^b	9	38	0.423	0.515^b
ER
-	13	13			3	23
+	29	27	0.023	0.880^d	14	42	1.958	0.162^d
PR
-	13	7			3	17
+	29	33	2.010	0.156^d	14	48	0.529	0.467^d
HER-2
-～+	32	33			14	51
++～+++	10	7	0.496	0.481^d	3	14	0.124	0.725^d

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