Abstract:

Objective

To explore the expressions of GST-π,P-gp and TOPO-Ⅱand their correlation with chemotherapy in primary breast cancer.

Methods

By using immunohistochemical S-P method, the expressions of GST-π,P-gp, and TOPO-Ⅱwere retrospectively compared and detected in 84 primary breast cancer cases:( the untreated group of 56 cases and the preoperative adjunctive chemotherapy group of 28 cases). The expressions of adjunctive pre- and post-chemotherapy of breast cancer in the 28 cases were also investigated. Results were analyzed using Chi square test for the numeration data of group design and paired design.

Results

The expressions of GSTπ, P-gp and TOPO-Ⅱin breast cancer tissues were not associated with pathological factors (P＞0.050). The positive expression rates of GST-π,P-gp and TOPO-Ⅱhad no statistical significance between the 28 cases with pre-chemotherapy and the 56 cases of untreated primary breast cancer, with the Chi-square value and P value of 0.45 and 0.504, 0. 41 and 0. 532, and 1. 20 and 0. 273, respectively; but had significant difference between prechemothrapy and postchemotherapy in the 28 cases, with the Chi-square value and p value of 4.57 and 0.033, 3.90 and 0.048, and 4.98 and 0.026, respectively; the positive expression rates of GST-π and P-gp of postchemotherapy increased significantly compared with pretchemotherapy, but the positive expression rate of TOPO-Ⅱshowed a decrease trend after chemotherapy.

Conclusion

Chemotherapy can affect the expressions of GST-π, P-gp and TOPO-Ⅱin primary breast cancer;monitoring their expressions in breast cancer has an important guiding significance.

Key words: GST-π, P-gp, TOPO-Ⅱ, Breast neoplasms, Immunohistochemistry, Multidrug resistance-associated proteins