Effect of mitochondrial DNA deletion on the sensitivity of MDA-MB-231 cells to chemotherapeutic drugs

doi:10.3877/cma.j.issn.1674-0807.2008.04.007

Abstract

Abstract:

Objective

To explore the relationship between mitochondrial DNA deletion and drug resistance in human breast cancer cells.

Methods

The rho⁰-derivative of MDA-MB-231 was generated by treating the cultured cells with ethidium bromide.The morphologies were confirmed by transmission electron microscope（TEM）.The sensitivities to chemotherapy were determined by Collagen Gel Droplet Embedded Culture-Drug Sensitivity Test（CD-DST）.P-glycoprotein（P-gp）and breast cancer resistance protein（BCRP） expression were detected by immunohistochemistry.All the differences were comparatively analyzed between MDA-MB-231 and rho⁰ MDA-MB-231 cells.

Results

Compared with MDA-MB-231 cells，rho⁰ MDAMB-231 cells showed different characters，including more mitochondria and cristae vacuolation，less drug sensitivity，and markedly higher expression of P-gp and BCRP.

Conclusions

Mitochondrial DNA deletion may play a role in the formation of drug resistance phenotype of human breast cancer.

Key words: Mitochondria, Mitochondrial DNA, Breast neoplasms, Multidrug resistance

Yu-lan SUN, Feng GU, Zu-ping ZHANG, Li Fu. Effect of mitochondrial DNA deletion on the sensitivity of MDA-MB-231 cells to chemotherapeutic drugs[J]. Chinese Journal of Breast Disease(Electronic Edition), 2008, 02(04): 429-435.

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URL: https://zhrxbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0807.2008.04.007

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Figures/Tables 4

References 19

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药物	浓度1/(μg·ml^-1)	浓度2/(μg·ml^-1)
顺铂	0.2	2.0
吉西他滨	0.03	0.30
紫杉醇	1	10
氟尿嘧啶	1	10
吡柔比星	1	10

药物	浓度1/(μg·ml^-1)	浓度2/(μg·ml^-1)
顺铂	0.2	2.0
吉西他滨	0.03	0.30
紫杉醇	1	10
氟尿嘧啶	1	10
吡柔比星	1	10

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