Abstract:

Objective

To explore the correlation of polymorphism of CYP1A1 gene MspⅠsite and CYP1A2 gene C734A site with breast cancer in women of Han nationality.

Methods

A case-control study was performed in 144 female patients with breast cancer (breast cancer group) and 152 healthy women (control group) in Sichuan Provincial People's Hospital from September 2011 to August 2012.Genotypes of CYP1A1 gene Msp Ⅰ and CYP1A2 gene C734A polymorphic sites were defined using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method.Chi-square test was used to compare the genotype and allele frequencies between breast cancer and control group.

Results

The T allele frequencies at MspⅠsite of CYP1A1 gene were 0.73 and 0.65 in breast cancer and control group, respectively, which showed a significant difference (χ² =4.94, P=0.03).Comparing C allele with T allele, the odds ratio of breast cancer risk was 0.67 (95%CI:0.47-0.96).C allele frequencies at the C734A site of CYP1A1 gene were 0.26 and 0.29 in breast cancer and control group, respectively.No significant difference was observed between those two groups (χ²=0.63, P=0.43).The patients in breast cancer group were further divided into subgroups according to ER and PR status.No significant difference of allele frequency at the 2 polymorphic sites was observed between the ER (+)/(-) or PR (+)/(-) subgroups, respectively [ER(+) subgroup vs ER(-)subgroup:χ²=0.34,0.01;PR(+) subroup vs PR(-) subgroup:χ²=0.60,0.68;all P＞0.05].

Conclusion

The polymorphism of CYP1A1 gene MspⅠsite is associated with breast cancer in women of Han nationality.

Key words: Breast neoplasms, Polymorphism, single nucleotide, Cytochrome P-450 CYP1A1, Cytochrome P-450 CYP1A2, Polymerase chain reaction-restriction fragment length polymorphism