Abstract:

Objective

To investigate the expression of survivin and proliferating cell nuclear antigen (PCNA)in breast cancer tissue and their clinical significance.

Methods

The clinical pathology data of 156 patients with breast cancer managed with a routine surgical treatment,who had no chemotherapy or radiation therapy before operation,in Zaozhuang Municipal Hospital from January 2001 to December 2006 were retrospectively analyzed .Immunohistochemical SP method was used to determine the expressions of survivin and PCNA in the cancer tissues.Comparison between qualitative data was performed using linear trend test,Kappa test or Chisquare test according to different analytical purposes respectively.Logistic regression analysis was applied for the analysis of positive expression-related factors of survivin and PCNA.

Results

The positive expressions of survivin and PCNA protein in breast cancer tissues were not related with pathology feature and tumor size (P＞0.050),but were associated with tumor histological grade,clinic stage and the number of lymph node metastasis (P＜0.050).The expressions of survivin and PCNA-positive cancer tissues were increased with the decrease of cancer tissue differentiation,and the increase in the number of lymph node metastasis and TNM staging.Logistic regression analysis showed that the histological grade of breast cancer was the main factor impacting the survivin and PCNA expression (P＜0.050).Survivin was positively related with PCNA (Kappa value,0.256;P＜0.001).Survivin and PCNA dual-positive patients had higher rates of recurrence and metastasis than survivin and PCNA dual-negative patients(χ²=6.510,P=0.011).

Conclusion

Detection of the expressions of survivin and PCNA can estimate the biological behavior and predict the prognosis for breast cancer.

Key words: Breast neoplasms;Immunohistochemistry;Survivin;PCNA;Prognosis