Abstract:

Objective

To explore in vitro chemosensitivity of breast cancer with TECIA (tissue culture-end point staining-computer image analysis)and its clinic value in chemotherapy of breast cancer.

Methods

Forty-six specimens of breast cancer were selected from the patients who underwent breast cancer surgery from September 2005 to September 2008 in our hospital and were used for evaluating the chemosensitivity of breast cancer cells with TECIA.The drugs tested were the drugs commonly used for breast cancer including paclitaxol (PTX),navelbine (NVB),(adriamycin)ADM,methotrexate (MTX), (5-fluorouracil)5-FU, (cisplatin )DDP,and combined regimens as TA (PTX+ADM),NP(NVB+DDP),CAF(CTX+ADM+5-FU)and CMF(CTX+ MTX+5-FU).The drug sensitivity difference was compared using Chi square test.

Results

In the 46 specimens of breast cancer,the sensitivity to PTX was 60.9%(28/46),to NVB 58.7%(27/46),to ADM 56.5%(27/46),to MTX 37.0%(17/46),to 5-FU 34.8%(16/46)and to DDP 26.1%(12/46).There was no difference in PTX,NVB,ADM and MTX.Also,there was no difference in 5-FU and DDP(χ²=6.724,P=0.081;χ²=0.821,P=0.365).The sensitivity of PTX and NVB was higher than that of MTX,5-FU and DDP(P ＜0.003).The sensitivity to TA,NP,CAF and CMF was 71.7%(33/46),67.4%(31/46),45.7%(21/46)and 39.1%(18/46),respectively.There was no difference between TA and NP and between CAF and CMF (P＞0.007).The sensitivity of TA and NP was higher than that of CAF and CMF,with statistical difference (P ＜0.007).The sensitivity of PTX and NVB or chemotherapy regimens with PTX and NVB was higher.There was individual difference in the sensitivity of chemotherapy drugs.

Conclusion

In vitro chemosensitivity of breast cancer with TECIA is of guiding significance in clinics.

Key words: Breast neoplasms;Drug screening test;Tissue culture-end point staining-computer image analysis;Individual chemotherpy