Abstract:

Objective

To clarify the relationships between ITGBL1(integrin beta-like 1)m RNA expression and clinicopathological factors in breast cancer,and to evaluate its clinincal value in predicting the prognosis of breast cancer.

Methods

Realtime reverse transcription-polymerase chain reaction (RT-PCR)was used to detect the expression level of ITGBL1 m RNA in 180 cases of primary invasive ductal carcinoma,who underwent radical mastectomy and were followed up for at least 3 years.Receiver Operating Characteristic(ROC)curve was used to identify the cut-off value of ITGBL1 mRNA level to group the patients.Chi-square test was used to compare differences between groups.Survival analysis was carried out using Kaplan-Meier analysis.Log-rank test was used to compare the differences of survival rates between groups.

Results

The high expression rate of ITGBL1 m RNA in ER-negative breast cancer patients was lower than in ER-positive breast cancer patients (χ²=7.475,P=0.006),lower in histological grade III breast cancer patients than in histological grades I and II breast cancer patients (χ²=4.410,P=0.036);No statistically significant difference was observed between groups in age,menostasis status,tumor size,lymph node status,clinical stage and the status of PR and HER-2(χ²=4.569,P=0.033).Three-year and 5-year disease-free survival and 5-year metastasis-free survival of cases had no statistical differences between groups.(P＞0.050),but with decrease of the ITGBL1 m RNA expression,there was a decrease trend in survival.

Conclusions

High expression level of ITGBL1 m RNA is one of the biological characteristics of ER-positive breast cancer.Patients with low expression of ITGBL1 m RNA have higher degree of malignancy,poor differentiation,and poor prognosis,indicating ITGBL1 m RNA is a potential molecular marker for predicting prognosis of breast cancer.

Key words: Breast neoplasms;Metastasis;ITGBL1;Quantitative RT-PCR