Obtaining cancer stem cells from MCF-7 breast cancer cell line with cloning cylin-der method

doi:10.3877/cma.j.issn.1674-0807.2009.02.009

Abstract

Abstract:

Objective

To isolate cancer stem-like cells from MCF-7 breast cancer cell line with modified cloning cylinder method and to test its feasibility.

Methods

Single cell cloning and stem cell screening with stem cell culture medium were applied to obtain sphere-like breast cancer stem cells.Immunofluorescence staining with CD44 and CD24 and inducement and differentiation experiment were performed.

Results

The proportion of CD44⁺CD24^-/Low cells was 12.1%,and the proportion in the holoclone was high.The majority of cells in culturing mammospheres were stained positively for CD44 and negatively for CD24.The mammospheres had abundant CD44⁺CD24^-/Low cells.Sphere-like breast cancer stem cells were obtained from holoclone cells,which were seeded at low density and suspension cultured with stem cell culture medium.When the sphere-like breast cancer stem cells were induced to differentiate,duct-like structures emerged and expressed CK18 and CK14.

Conclusion

The cloning cylinder method is a simple and effective way to obtain breast cancer stem celllike cells from MCF-7 breast cancer cell lines.

Key words: Breast neoplasms, Tumor stem cells, Cloning cylinder method

Jing XIE, Yan ZHOU, Peng TANG, Ling ZHONG, Jun JIANG. Obtaining cancer stem cells from MCF-7 breast cancer cell line with cloning cylin-der method[J]. Chinese Journal of Breast Disease(Electronic Edition), 2009, 03(02): 187-194.

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URL: https://zhrxbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0807.2009.02.009

https://zhrxbzz.cma-cmc.com.cn/EN/Y2009/V03/I02/187

Figures/Tables 10

References 7

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