Proteome analysis for the identification of tumor-associated biomarkers to predict breast cancer prognosis

doi:10.3877/cma.j.issn.1674-0807.2007.03.013

Abstract

Abstract:

Objective

To find new biomarkers that can estimate the prognosis of breast cancer patients，for the patients who have the similar staging and accept same treatment get different treatment results.

Methods

Protein expression differences of serum samples of 64 breast cancer patients were analyzed with IMAC-3 and WCX-2 Ciphergen Protein Chip Arrays.Five-year follow-up of all the 64 breast cancer patients showed 13 patients died.The protein atlas of the 13 dead patients was compared with that of the other patients.

Results

On WCX-2 chip，a panel of two proteins（M_r9405 and M_r6424）was selected based on their collective contribution to the optimal separation between the dead patients and alive patients after 5-year follow-up.On M_r9405 site survived breast cancer patients had low expression，on M_r6424 site survived breast cancer patients had high expression，with sensitivity of 76.9%-84.6%and specificity of 76.4%-80.3%.On IMAC-3 chip，another two proteins（M_r4643 and M_r5910）had the ability to distinguish between the dead patients and alive patients after 5-year follow-up.Survived breast cancer patients had high expression，with sensitivity of 61.5%～76.9%and specificity of 68.6%～74.5%.

Conclusion

Protein expression in dead patients after 5-year follow-up is different from that of alive patients after 5-year follow-up，and those proteins with different expressions can be used as new biomarkers to predict breast cancer prognosis.

Key words: Breast neoplasms, Proteome analysis

Xi WANG, Ming-tian YANG, Ze-ming XIE, Jun TANG, Zhong-mei ZHOU, Yi-xin ZENG, Zhen-yu ZHU. Proteome analysis for the identification of tumor-associated biomarkers to predict breast cancer prognosis[J]. Chinese Journal of Breast Disease(Electronic Edition), 2007, 01(03): 40-43.

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URL: https://zhrxbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0807.2007.03.013

https://zhrxbzz.cma-cmc.com.cn/EN/Y2007/V01/I03/40

Figures/Tables 1

References 14

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蛋白质芯片	蛋白相对分子质量	敏感性/（%）	特异性/（%）
WCX-2	9405	76.9（10/13）	80.3（41/51）
	6424	84.6（11/13）	76.4（39/51）
IMAC-3	4643	61.5（8/13）	68.6（35/51）
	5910	76.9（10/13）	74.5（38/51）

蛋白质芯片	蛋白相对分子质量	敏感性/（%）	特异性/（%）
WCX-2	9405	76.9（10/13）	80.3（41/51）
	6424	84.6（11/13）	76.4（39/51）
IMAC-3	4643	61.5（8/13）	68.6（35/51）
	5910	76.9（10/13）	74.5（38/51）

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