Experimental research of targeting hTERT gene inhibited in breast cancer therapy by RNA interference

doi:10.3877/cma.j.issn.1674-0807.2007.01.013

Abstract

Abstract:

Objective

To investigate the feasibility and specificity of breast cancer gene therapy，we utilized RNAi to inhibit hTERT gene expression in MCF-7 cells of breast cancer.

Methods

Small intererence RNA （siRNA） homologous to hTERT gene （hTERT-siRNA） was recombined with pGenesil-1 vector， then transfected it into MCF-7 cells of breast cancer to induce RNAi， and then detected hTERT gene expression alterations and tumor cell proliferation in both hTERT-siRNA treatment groups and control groups by MTT assay， RT-PCR and Western blot.

Results

The expression of hTERT had been obviously inhibited by siRNA.The inhibition rate of cell proliferation was 43%.The mRNA level of hTERT was obviously decreased， and its protein expression reduced by 39.8%.

Conclusions

siRNA-hTERT effectively inhibited hTERT gene expression and breast cancer cell proliferation in vitro.

Key words: RNAi, Breast cancer, hTERT

Xiaohua Zeng, Chang'an Liu, Jianchuan Deng. Experimental research of targeting hTERT gene inhibited in breast cancer therapy by RNA interference[J]. Chinese Journal of Breast Disease(Electronic Edition), 2007, 01(01): 39-42.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhrxbzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-0807.2007.01.013

https://zhrxbzz.cma-cmc.com.cn/EN/Y2007/V01/I01/39

Figures/Tables 3

References 11

［1］	Hiyama E， Gollahon L， Kataoka T，etal.Telomerase activity in human breast tumors［J］.J Natl Cancer Inst，1996，88（2）：116 -122.
［2］	Kolquist K A， Ellisen L W， Counter C M，etal.Expression of TERT in early premalignant lesions and a subset of cells in normal tissues［J］.Nat Genet，1998，19（2）：182 -186.
［3］	Sui G， Soohoo C， Affarel B， etal.A DNA vectorbased RNAi technology to suppress gene expression in mammalian cells ［J］.Proc Natl Acad Sci， 2002，99（8）：5515 -5520.
［4］	Zou L， Luo C L， Tu Z G，etal.Expression of hTERT and c-myc mRNA in transitional cell carcinoma of the bladder［J］.Chinese Medical J，2002，16（5）：103 -106.
［5］	Shay J W， Van Der Haegen B A， Ying Y，etal.The frequency of immortalization of human fibroblasts and mammary epithelial cells transfected with SV40 large T-antigen［J］.Exp Cell Res，1993，209（1）：45-52.
［6］	Izbicka E， Wheelhouse R T， Raymond E， etal.Effects of cationic porphyrins as G-quadruplex interactive agents in human tumor cells［J］.Cancer Res，1999，59（3）：639 -644.
［7］	Melana S M， Holland J F， Pogo B G.Inhibition of cell growth and telomerase activity of breast cancer cells in vitro by 3-'azido-3-'deoxythymidine［J］.Clin Cancer Res，1998，4（3）：493 -696.
［8］	Wilda M， Fuchs U， Wossmann W， etal.Killing of leukemic cells with a BCR/ABL fusion gene by RNA interference （RNAi）［J］.Oncogene，2002，21（37）：5716 - 5724.
［9］	Barnor J S，Miyano-Kurosaki N，Yamaguchi K，etal.Lentiviral-mediated delivery of combined HIV-1 decoy TAR and Vif siRNA as a single RNA molecule that cleaves to inhibit HIV-1 in transduced cells［J］.Nucleosides Nucleotides Nucleic Acids， 2005， 24 （5）：431 - 434.
［10］	Shlomai A， Shaul Y.Inhibition of hepatitis B virus expression replication by RNA interference［J］.Hepatology，2003，37（4）：764-770.
［11］	Kapadia S B， Brideau-Andersen A， Chisari F V.Interference of hepatitis C virus RNA replication by short interfering RNAs［J］.Proc Natl Acad Sci， 2003，100（4）：2014 -2018.

[1]	Wei Hong, Xirong Ye, Zhihong Liu, Yinfeng Yang, Zhihong Lyu. Value of ultrasound radiomics combined with clinicopathological features in predicting complete pathological response to neoadjuvant chemotherapy for breast cancer [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2024, 21(06): 571-579.
[2]	Yang Zhao, Yingcong Xiao, Yan Ju, Xiaozhi Dang, Linli Cai, Wenxin Xue, Yang Li, Yao Xiao, Yuqi Guo, Hongping Song. Clinical value of automated breast ultrasound combined with immunohistochemistry in early prediction of pathological complete response to neoadjuvant chemotherapy in breast cancer [J]. Chinese Journal of Medical Ultrasound (Electronic Edition), 2024, 21(04): 361-369.
[3]	Wei Liu, Yunfeng Niu, Jie An. LINC01232 promotes malignant progression of triple negative breast cancer through miR-516a-5p/BCL9 axis [J]. Chinese Journal of Breast Disease(Electronic Edition), 2024, 18(06): 330-338.
[4]	Haigang Niu, Wenke Guo. Expressions of dual specific phosphatase 14 and nuclear receptor interacting protein 1 in triple negative breast cancer and their prognostic value [J]. Chinese Journal of Breast Disease(Electronic Edition), 2024, 18(04): 199-205.
[5]	Zhao Zhang, Chengyu Luo, Shuqi Zhang, Ping He, Xubin Li. Comparison of the effect, complication rate and recurrence rate of different operation methods in treating early breast cancer [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(05): 494-497.
[6]	Jia Song, Bo Wang, Kailu Sun, Jiangfeng Shang, Danping Wu, Yi Zhao. Application of indocyanine green fluorescence imaging combined with methylene blue staining in sentinel lymph node biopsy of breast cancer [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(05): 498-501.
[7]	Jianna Sun, Lingjun Kong, Chongxi Ren, Kun Mu, Xiaorui Wang. Prognostic analysis of 266 patients with stage IV breast cancer undergoing surgery for the first time [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(05): 502-505.
[8]	Danping Tang, Ping Wang, Mengdie Jiang, Xiaorong Yang. Research progress of autologous fat transplantation in breast reconstruction after breast cancer [J]. Chinese Journal of Operative Procedures of General Surgery(Electronic Edition), 2024, 18(05): 582-585.
[9]	Qi Liu, Shoukai Wang, Shuai Wang, Yuqing Su, Zhuang Ma, Haijun Chen, Pilei Si. Advances in research of intratumoral microbiome in breast cancer [J]. Chinese Journal of Clinicians(Electronic Edition), 2024, 18(09): 841-845.
[10]	Junwei Cui, Huali Cai, Yibin Hu, Hui Hu. Clinical value of methylene blue combined with marker clip and hook-wire localization in evaluation of axillary lymph node metastasis after neoadjuvant chemotherapy for breast cancer [J]. Chinese Journal of Clinicians(Electronic Edition), 2024, 18(07): 625-632.
[11]	Yuying Wang, Shiwei Liu, Rui Wang, Yaling Zng, Xihui Tu, Purong Zhang. Predictive factors for pathological complete response to neoadjuvant therapy for breast cancer in elderly patients [J]. Chinese Journal of Clinicians(Electronic Edition), 2024, 18(07): 641-646.
[12]	Shuai Wang, Zhiyuan Zhang, Yuqing Su, Wenwen Li, Shoukai Wang, Qi Liu, Wentao Li. Mendelian randomization and its application in breast cancer research [J]. Chinese Journal of Clinicians(Electronic Edition), 2024, 18(07): 671-676.
[13]	Mengting Zhang, Lamu Qiong, Zhen Se, Yiqun Li, Wangmu DeQing. Neoadjuvant chemotherapy for breast cancer patients in Tibet: a real-world study [J]. Chinese Journal of Clinicians(Electronic Edition), 2024, 18(05): 441-446.
[14]	Yang Zhao, Yingcong Xiao, Yan Ju, Xiaozhi Dang, Linli Cai, Wenxin Xue, Yang Li, Yao Xiao, Yuqi Guo, Hongping Song. Clinical value of a nomogram model based on automated breast ultrasound in early prediction of pathological complete response to neoadjuvant chemotherapy in HER-2 positive breast cancer [J]. Chinese Journal of Clinicians(Electronic Edition), 2024, 18(04): 355-362.
[15]	Hongshan Huang, Chengcai Chen. Progress in breast cancer sentinel lymph node detection by translymphatic contrast-enhanced ultrasound [J]. Chinese Journal of Clinicians(Electronic Edition), 2024, 18(04): 411-414.

Please choose a citation manager

Content to export