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中华乳腺病杂志(电子版) ›› 2022, Vol. 16 ›› Issue (06) : 376 -379. doi: 10.3877/cma.j.issn.1674-0807.2022.06.008

综述

一步核酸扩增技术在乳腺癌腋窝淋巴结转移评估中的应用
韩春光1, 陆晨阳2, 裴静3,()   
  1. 1. 230022 合肥,安徽医科大学第一附属医院小儿外科;230022 合肥,安徽医科大学第一附属医院普通外科
    2. 230022 合肥,安徽医科大学第一附属医院内分泌科
    3. 230022 合肥,安徽医科大学第一附属医院普通外科;230022 合肥,安徽医科大学第一附属医院乳腺外科
  • 收稿日期:2021-01-06 出版日期:2022-12-01
  • 通信作者: 裴静
  • 基金资助:
    安徽省自然科学基金资助项目(2008085MH295); 安徽医科大学第一附属医院临床研究项目(LCYJ2021YB008)

Application of one-step nucleic acid amplification in assessing axillary lymph node metastasis in breast cancer

Chunguang Han1, Chenyang Lu2, Jing Pei3()   

  • Received:2021-01-06 Published:2022-12-01
  • Corresponding author: Jing Pei
引用本文:

韩春光, 陆晨阳, 裴静. 一步核酸扩增技术在乳腺癌腋窝淋巴结转移评估中的应用[J]. 中华乳腺病杂志(电子版), 2022, 16(06): 376-379.

Chunguang Han, Chenyang Lu, Jing Pei. Application of one-step nucleic acid amplification in assessing axillary lymph node metastasis in breast cancer[J]. Chinese Journal of Breast Disease(Electronic Edition), 2022, 16(06): 376-379.

一步核酸扩增(OSNA)是一种针对细胞角蛋白19(CK19 mRNA的快速半自动检测技术,可用于乳腺癌腋窝淋巴结转移的评估。目前OSNA已经在乳腺癌术中前哨淋巴结转移评估、非前哨淋巴结转移评估、pN2~3腋窝淋巴结转移风险预测和一种新的基于分子的乳腺癌淋巴结分期方法[pN(mol)分类]等领域显示出了确切的临床应用价值,对于避免二次腋窝淋巴结清扫术或过度的腋窝淋巴结清扫术具有重要意义。本文综述了OSNA在乳腺癌腋窝淋巴结转移评估中的应用和研究进展,总结了这一检测方法的未来应用前景和发展方向。

图1 一步核酸扩增技术检测原理和流程示意图 a图为一步核酸扩增技术检测流程;b图为一步核酸扩增技术检测技术反应体系构成;c图为一步核酸扩增技术检测分析系统注:SLNB为前哨淋巴结活检;ALND为腋窝淋巴结清扫;OSNA为一步核酸扩增技术;RT-LAMP为逆转录环介导等温扩增技术;FIP为正向内引物;BIP为反向内引物;F1、F2、F3、B1、B2、B3为靶基因上的6个特异性片段,是引物的识别位点
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