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中华乳腺病杂志(电子版) ›› 2022, Vol. 16 ›› Issue (06) : 376 -379. doi: 10.3877/cma.j.issn.1674-0807.2022.06.008

综述

一步核酸扩增技术在乳腺癌腋窝淋巴结转移评估中的应用
韩春光1, 陆晨阳2, 裴静3,()   
  1. 1. 230022 合肥,安徽医科大学第一附属医院小儿外科;230022 合肥,安徽医科大学第一附属医院普通外科
    2. 230022 合肥,安徽医科大学第一附属医院内分泌科
    3. 230022 合肥,安徽医科大学第一附属医院普通外科;230022 合肥,安徽医科大学第一附属医院乳腺外科
  • 收稿日期:2021-01-06 出版日期:2022-12-01
  • 通信作者: 裴静
  • 基金资助:
    安徽省自然科学基金资助项目(2008085MH295); 安徽医科大学第一附属医院临床研究项目(LCYJ2021YB008)

Application of one-step nucleic acid amplification in assessing axillary lymph node metastasis in breast cancer

Chunguang Han1, Chenyang Lu2, Jing Pei3()   

  • Received:2021-01-06 Published:2022-12-01
  • Corresponding author: Jing Pei
引用本文:

韩春光, 陆晨阳, 裴静. 一步核酸扩增技术在乳腺癌腋窝淋巴结转移评估中的应用[J/OL]. 中华乳腺病杂志(电子版), 2022, 16(06): 376-379.

Chunguang Han, Chenyang Lu, Jing Pei. Application of one-step nucleic acid amplification in assessing axillary lymph node metastasis in breast cancer[J/OL]. Chinese Journal of Breast Disease(Electronic Edition), 2022, 16(06): 376-379.

一步核酸扩增(OSNA)是一种针对细胞角蛋白19(CK19 mRNA的快速半自动检测技术,可用于乳腺癌腋窝淋巴结转移的评估。目前OSNA已经在乳腺癌术中前哨淋巴结转移评估、非前哨淋巴结转移评估、pN2~3腋窝淋巴结转移风险预测和一种新的基于分子的乳腺癌淋巴结分期方法[pN(mol)分类]等领域显示出了确切的临床应用价值,对于避免二次腋窝淋巴结清扫术或过度的腋窝淋巴结清扫术具有重要意义。本文综述了OSNA在乳腺癌腋窝淋巴结转移评估中的应用和研究进展,总结了这一检测方法的未来应用前景和发展方向。

图1 一步核酸扩增技术检测原理和流程示意图 a图为一步核酸扩增技术检测流程;b图为一步核酸扩增技术检测技术反应体系构成;c图为一步核酸扩增技术检测分析系统注:SLNB为前哨淋巴结活检;ALND为腋窝淋巴结清扫;OSNA为一步核酸扩增技术;RT-LAMP为逆转录环介导等温扩增技术;FIP为正向内引物;BIP为反向内引物;F1、F2、F3、B1、B2、B3为靶基因上的6个特异性片段,是引物的识别位点
[1]
Belmonte R, Messaggi-Sartor M, Ferrer M, et al. Prospective study of shoulder strength, shoulder range of motion, and lymphedema in breast cancer patients from pre-surgery to 5 years after ALND or SLNB [J]. Support Care Cancer201826(9):3277-3287.
[2]
Jimbo K, Kinoshita T, Ogura T, et al. Prediction score model for non-sentinel and four or more nodal metastases using a combined method of one-step nucleic acid amplification and histology in sentinel node-positive breast cancer patients [J]. Eur J Surg Oncol, 2020, 46(4 Pt A):516-521.
[3]
Bevers TB, Helvie M, Bonaccio E, et al. Breast cancer screening and diagnosis, version 3.2018, NCCN clinical practice guidelines in oncology [J]. J Natl Compr Canc Netw, 2018, 16(11):1362-1389.
[4]
van de Vrande S, Meijer J, Rijnders A, et al. The value of intraoperative frozen section examination of sentinel lymph nodes in breast cancer [J]. Eur J Surg Oncol, 2009, 35(3):276-280.
[5]
Tew K, Irwig L, Matthews A, et al. Meta-analysis of sentinel node imprint cytology in breast cancer [J]. Br J Surg, 2005, 92(9):1068-1080.
[6]
Orsaria P, Caredda E, Genova F, et al. Additional nodal disease prediction in breast cancer with sentinel lymph node metastasis based on clinicopathological features [J]. Anticancer Res, 2018, 38(4):2109-2117.
[7]
赵彬,范林军. 乳腺癌淋巴结转移术中检测新进展:一步法核酸扩增技术 [J/CD]. 中华乳腺病杂志(电子版), 2009, 3(2): 245-249.
[8]
Notomi T, Okayama H, Masubuchi H, et al. Loop-mediated isothermal amplification of DNA [J]. Nucleic Acids Res, 2000, 28(12):E63.
[9]
Gradishar WJ, Anderson BO, Abraham J, et al. Breast cancer, version 3.2020, NCCN clinical practice guidelines in oncology [J]. J Natl Compr Canc Netw, 2020, 18(4):452-478.
[10]
Visser M, Jiwa M, Horstman A, et al. Intra-operative rapid diagnostic method based on CK19 mRNA expression for the detection of lymph node metastases in breast cancer [J]. Int J Cancer, 2008, 122(11):2562-2567.
[11]
Jimbo K, Kinoshita T, Suzuki J, et al. Sentinel and nonsentinel lymph node assessment using a combination of one-step nucleic acid amplification and conventional histological examination [J]. Breast, 2013, 22(6):1194-1199.
[12]
Cong Y, Wang S, Zou H, et al. Imaging predictors for nonsentinel lymph node metastases in breast cancer patients [J]. Breast Care (Basel), 2020, 15(4):372-379.
[13]
Guo X, Liu Z, Sun C, et al. Deep learning radiomics of ultrasonography: Identifying the risk of axillary non-sentinel lymph node involvement in primary breast cancer [J]. EBioMedicine, 2020, 60:103 018.
[14]
Sa-Nguanraksa D, O-Charoenrat E, Kulprom A, et al. Nomogram to predict non-sentinel lymph node status using total tumor load determined by one-step nucleic acid amplification: first report from Thailand [J]. Breast Cancer, 2019, 26(4):471-477.
[15]
Shimazu K, Sato N, Ogiya A, et al. Intraoperative nomograms, based on one-step nucleic acid amplification, for prediction of non-sentinel node metastasis and four or more axillary node metastases in breast cancer patients with sentinel node metastasis [J]. Ann Surg Oncol, 2018, 25(9):2603-2611.
[16]
Shimazu K, Miyake T, Okuno J, et al. One-step nucleic acid amplification can identify sentinel node-negative breast cancer patients with excellent prognosis [J]. Anticancer Res, 2019, 39(3):1447-1454.
[17]
Osako T, Iwase T, Ushijima M, et al. A new molecular-based lymph node staging classification determines the prognosis of breast cancer patients [J]. Br J Cancer, 2017, 117(10):1470-1477.
[18]
Shirzadi A, Mahmoodzadeh H, Qorbani M. Assessment of sentinel lymph node biopsy after neoadjuvant chemotherapy for breast cancer in two subgroups: Initially node negative and node positive converted to node negative-a systemic review and meta-analysis [J]. J Res Med Sci, 2019, 24:18.
[19]
Montagna G, Corso G, Di Micco R, et al. Axillary management after neoadjuvant treatment [J]. Minerva Chir, 2020, 75(6):400-407.
[20]
Mariscal Martínez A, Vives Roselló I, Salazar Gómez A, et al. Advantages of preoperative localization and surgical resection of metastatic axillary lymph nodes using magnetic seeds after neoadjuvant chemotherapy in breast cancer [J]. Surg Oncol, 2021, 36:28-33.
[21]
Damin AP, Zancan M, Melo MP, et al. Sentinel lymph node biopsy after neoadjuvant chemotherapy in patients with node-positive breast cancer: guiding a more selective axillary approach [J]. Breast Cancer Res Treat, 2021, 186(2):527-534.
[22]
Liu LC, Lang JE, Lu Y, et al. Intraoperative frozen section analysis of sentinel lymph nodes in breast cancer patients: a meta-analysis and single-institution experience [J]. Cancer, 2011, 117(2):250-258.
[23]
Vieites B, López-García Castilla C, et al. CK19 expression in breast tumours and lymph node metastasis after neoadjuvant therapy [J]. Histopathology, 2016, 69(2):239-249.
[24]
Osako T, Iwase T, Kimura K, et al. Intraoperative molecular assay for sentinel lymph node metastases in early stage breast cancer: a comparative analysis between one-step nucleic acid amplification whole node assay and routine frozen section histology [J]. Cancer, 2011, 117(19):4365-4374.
[25]
Espinosa-Bravo M, Navarro-Cecilia J, Ramos Boyero M, et al. Intraoperative assessment of sentinel lymph node by one-step nucleic acid amplification in breast cancer patients after neoadjuvant treatment reduces the need for a second surgery for axillary lymph node dissection [J]. Breast, 2017, 31:40-45.
[26]
von Minckwitz G, Untch M, Blohmer JU, et al. Definition and impact of pathologic complete response on prognosis after neoadjuvant chemotherapy in various intrinsic breast cancer subtypes [J]. J Clin Oncol, 2012, 30(15):1796-1804.
[27]
《乳腺癌新辅助治疗的病理诊断专家共识(2020版)》编写组.乳腺癌新辅助治疗的病理诊断专家共识(2020版)[J].中华病理学杂志202049(4):296-304.
[28]
Osako T, Tsuda H, Horii R, et al. Molecular detection of lymph node metastasis in breast cancer patients treated with preoperative systemic chemotherapy: a prospective multicentre trial using the one-step nucleic acid amplification assay [J]. Br J Cancer, 2013, 109(6):1693-1698.
[29]
Imeokparia FO, Hughes TM, Dossett LA, et al. Axillary pathologic complete response in inflammatory breast cancer patients: implications for SLNB? [J].Ann Surg Oncol, 2019, 26(10):3374-3379.
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