目的

探讨乳腺小黏蛋白(small breast epithelial mucin,SBEM)mRNA 作为检测乳腺癌患者外周血微转移指标的临床意义。

方法

用巢式逆转录-聚合酶链反应(Nested-RT-PCR)法检测22 例女性胃癌、16 例结/直肠癌、107 例乳腺癌、40 例乳腺纤维腺瘤患者和27 例健康志愿者外周血中SBEM-mRNA 的表达情况。

结果

107 例乳腺癌患者外周血中SBEM-mRNA 有57 例表达,阳性率为53.27%,其余对照组中SBEM-mRNA 表达均为阴性。 两组比较差异有统计学意义(P ＜0. 05)。SBEM-mRNA 的阳性表达与患者的淋巴结转移状况和TNM 分期均有相关性(P 均＜0.01);而与患者的年龄、原发肿瘤大小、病理类型、ER、PR 等无相关性(P ＞0.05)。

结论

SBEM-mRNA 特异表达于乳腺癌患者的外周血,可能成为检测乳腺癌患者外周血中微转移和判断预后的指标之一。

Objective

To evaluate the clinical significance of SBEM (small breast epithelial mucin) mRNA as a micrometastases marker in peripheral blood of breast cancer.

Methods

The SBEMmRNA level in peripheral blood was detected in 22 patients with gastric cancer,16 colon/rectal carcinoma,107 breast cancer,40 breast fibroadenoma and 27 healthy volunteers as controls using rested reverse transcription-polymerase chain reaction (Nested-RT-PCR).

Results

The positive expression rate of SBEMmRNA in peripheral blood of breast cancer patients was 53.27%(57/107 cases),and no positive SBEMmRNA was found in other controls.There was a significant difference between the two groups (P ＜0.05).SBEM-mRNA expression was correlated with axillary lymph nodes status and TNM (P ＜0. 01), but uncorrelated with other clinical parameters (age,tumor size,histopathlogy types,estrogen receptor and progesterone receptor).

Conclusion

SBEM-mRNA is sensitive and specific for breast cancer,and the positive expression in peripheral blood may be a marker in detecting micrometastases and judging prognosis.