目的

探讨乳腺癌组织中细胞周期蛋白D1(cyclin D1)的表达水平与腋窝淋巴结转移状态的关系。

方法

回顾性收集解放军总医院乳腺专病中心2014 年全年收治的1 012 例乳腺癌患者资料,患者分别编号。 采用免疫组织化学的方法检测术后癌组织中cyclin D1 的表达水平,并根据其表达水平将患者分为5 组,分别为1 组(cyclin D1 阴性)、2 组(cyclin D1≥1%且＜25%),3 组(cyclin D1≥25%且＜50%)、4 组(cyclin D1≥50%且＜75%)及5 组(cyclin D1≥75%),利用抽签的方法从每组分别抽取90 例患者作为受试对象,分析各组受试对象的腋窝淋巴结状态。 采用χ² 检验比较各组间腋窝淋巴结转移率是否具有差异,率的多重比较采用χ² 分割法。

结果

1 ～5 组患者的腋窝淋巴结转移率分别为40.0%、43.3%、35.6%、42.2%、66.7%,组间比较,差异有统计学意义(χ²=21.503,P＜0.001),进一步分析发现,cyclin D1 表达水平≥75%组的腋窝淋巴结转移率明显高于其他各组(χ² =12.857、9.899、17.431、10.841,P 均＜0.004)。

结论

当患者乳腺癌组织中cyclin D1 的表达水平超过参考值75%时,可以作为腋窝淋巴结转移的预测指标。

Objective

To detect the relationship between cyclin D1 expression in breast cancer tissue and axillary lymph node metastasis.

Methods

We labeled 1 012 breast cancer patients treated in Breast Disease Center, General Hospital of PLA in 2014 and detected the expression level of cyclin D1 in postoperative carcinoma tissues using immunohistochemical method. According to the cyclin D1 level, the patients were divided into 5 groups, namely group 1 (cyclin D1 negative), group 2 (cyclin D1≥1% and ＜25%), group 3(cyclin D1≥25% and ＜50%), group 4 (cyclin D1≥50% and ＜75%) and group 5 (cyclin D1≥75%).Ninety cases were randomized as subjects in each group and their lymph node status were analyzed. The axillary lymph node metastasis rates between groups were compared by χ² test. Pairwise comparison was performed by partition of χ² test.

Results

The rate of ymph node metastasis was 40.0%, 43.3%, 35.6%, 42.2% and 66.7% in groups 1-5 respectively, which showed a significant difference between groups(χ² =21.503,P＜0.001).The rate of lymph node metastasis in group 5 was significantly higher than that in other 4 groups (χ²=12.857, 9.899,17.431,10.841; all P＜0.004).

Conclusion

When the level of cyclin D1 in breast cancer tissue surpasses the reference value (75%), cyclin D1 can be regarded as a predictive factor of axillary lymph node metastasis.