目的

探讨乳腺原发癌组织中整合素β 样1(integrin bate-like 1,ITGBL1)m RNA 水平与临床病理因素的关系,评估其对预测患者预后的临床价值。

方法

采用实时定量RT-PCR 方法检测180例行乳腺癌根治术并随访3年以上的乳腺浸润性导管癌组织中ITGBL1 m RNA 的水平。采用受试者工作特征(receiver operating characteristic,ROC)曲线确定ITGBL1 mRNA 水平分组的阈值;用χ² 检验比较组间差异;用Kaplan-Meier法绘制生存曲线;用Log-rank时序检验比较组间生存期的差异。

结果

ER 阴性乳腺癌患者的ITGBL1 mRNA 高表达率低于ER 阳性患者(χ²=7.475,P=0.006),组织学Ⅲ级的乳腺癌患者ITGBL1 m RNA 高表达率低于Ⅰ～Ⅱ级乳腺癌患者(χ²=4.410,P=0.036),而在不同年龄和绝经状态、肿瘤大小、淋巴结转移状态、临床分期以及PR 和HER-2 状态各组间比较差异无统计学意义(P＞0.050)。ITGBL1 mRNA 低表达组患者3年无转移生存率低于高表达组,差异有统计学意义(χ²=4.569,P=0.033);3年无病生存率、5年无病生存率和5年无转移生存率组间比较虽差异无统计学意义(P＞0.050),但随ITGBL1 m RNA 表达降低而有下降趋势。

结论

乳腺原发癌中ITGBL1 m RNA 高表达是ER 阳性乳腺癌的生物学特征之一;ITGBL1 m RNA 低表达的乳腺癌恶性程度高、组织分化差且患者预后不良,提示ITGBL1基因是潜在的乳腺癌预后预测分子标志物。

Objective

To clarify the relationships between ITGBL1(integrin beta-like 1)m RNA expression and clinicopathological factors in breast cancer,and to evaluate its clinincal value in predicting the prognosis of breast cancer.

Methods

Realtime reverse transcription-polymerase chain reaction (RT-PCR)was used to detect the expression level of ITGBL1 m RNA in 180 cases of primary invasive ductal carcinoma,who underwent radical mastectomy and were followed up for at least 3 years.Receiver Operating Characteristic(ROC)curve was used to identify the cut-off value of ITGBL1 mRNA level to group the patients.Chi-square test was used to compare differences between groups.Survival analysis was carried out using Kaplan-Meier analysis.Log-rank test was used to compare the differences of survival rates between groups.

Results

The high expression rate of ITGBL1 m RNA in ER-negative breast cancer patients was lower than in ER-positive breast cancer patients (χ²=7.475,P=0.006),lower in histological grade III breast cancer patients than in histological grades I and II breast cancer patients (χ²=4.410,P=0.036);No statistically significant difference was observed between groups in age,menostasis status,tumor size,lymph node status,clinical stage and the status of PR and HER-2(χ²=4.569,P=0.033).Three-year and 5-year disease-free survival and 5-year metastasis-free survival of cases had no statistical differences between groups.(P＞0.050),but with decrease of the ITGBL1 m RNA expression,there was a decrease trend in survival.

Conclusions

High expression level of ITGBL1 m RNA is one of the biological characteristics of ER-positive breast cancer.Patients with low expression of ITGBL1 m RNA have higher degree of malignancy,poor differentiation,and poor prognosis,indicating ITGBL1 m RNA is a potential molecular marker for predicting prognosis of breast cancer.