目的

探讨p21^waf1基因转染对人乳腺癌细胞增殖活性影响及意义。

方法

应用脂质体介导法将pIRES-p21^waf1真核表达载体转染入人乳腺癌MCF-7 细胞系,并得到稳定的表达。 通过荧光定量PCR、Western blot 法分别检测p21^waf1基因表达及蛋白表达情况, MTT 法检测细胞增殖,流式细胞仪检测细胞周期分布。

结果

p21^waf1基因转染后乳腺癌细胞生长显著受抑制,细胞周期停滞于G₁ 期,转染组G₁ 期、S 期和G₂/M 期细胞比例分别为(70.52 ±3.21)%、(16.38 ±1.17)%、(9.42 ±0.98)%,未转染组则为(49.84 ±1.58)%、(36.83 ±1.36)%、(15.64 ±1.09)%,二者相比差异有统计学意义。

结论

p21^waf1基因转染能够抑制人乳腺癌细胞周期演进和增殖活性,进而阻止乳腺癌的发生、发展,为乳腺癌治疗的一种新手段。

Objective

To explore the role of p21^waf1 gene transfection in the proliferation of human breast carcinoma cells.

Methods

p21^waf1 gene packaged with liposome was transfected into the cells of a human breast carcinoma cell line MCF-7,which stably expressing p21^waf1gene. The cell growth was detected by MTT. The cell cycle pattern was assayed by flowcytometry.

Results

After transfection of pIRES-p21^waf1,p21^waf1 mRNA and the protein of MCF-7-p21^waf1 were highly expressed compared with control. The cell growth was obviously inhibited and resulted in an accumulation of cells in G₁,presenting that the proportion of the cells in G phase was obviously increased from 49.84 ±1.58% to 70.52 ±3.21%, and that of the cells in S and G₂/M phases was obviously decreased from 36.83 ±1.36% to 16.38 ±1.17% and from 15.64 ±1.09% to 9.42±0.98%, respectively, as compared with the control group.

Conclusion

p21^waf1gene transfection can inhibit the growth of human breast carcinoma cells and block the cell cycle, which then inhibit the genesis and development of breast cancer. So it may become a new method to treat breast cancer.