目的

研究乳腺癌MDA-MB-231 细胞在不同硬度基质上黏附率、增殖能力及整合素(intergrin)β1 表达变化的差异,探讨肿瘤细胞的生物学性状和细胞外基质硬度的相关性。

方法

将乳腺癌MDA-MB-231 细胞分别种植到不同硬度的人工模拟基质上(硬度分别为1、20 、40 kPa),3 d 后观察比较MDA-MB-231 细胞克隆形成差异。 用四唑盐(MTT)比色试验观察MDA-MB-231 细胞增殖活性的变化。 Western blot 检测细胞Intergrin β1 蛋白表达的变化。

结果

在硬度40 kPa 的人工基质上,乳腺癌细胞增殖能力、克隆形成能力及Intergrin β1 蛋白表达均高于其它两组,且差异有统计学意义。

结论

MDA-MB-231 细胞更容易在硬性较高的基质上增殖,其机制需进一步探讨。

Objective

To observe the proliferation ability,cell clone formation and intergrin β1 protein expression of MDA-MB-231 cell line on different matrixes and to explore relevance between biological behavior of MDA-MB-231 cell and hardness of extracellular matrix.

Methods

MDA-MB-231 cell was planted to different hardness of matrix (1,20,40 kPa respectively). The influence of different hardness of matrix on proliferation of MDA-MB-231 cell was detected by MTT test. The expression of intergrin β1 protein was determined by Western blot.Cell clone formation was observed by microscopy.

Results

The expression level of intergrin β1 protein and the proliferation ability of MDA-MB-231 cell on 40 kPa matrix were higher than other groups. The number of cell clones was greater than that formed in soft matrix.

Conclusions

MDA-MB-231 cell is more likely to growth on hard matrix. The hardness of matrix may be involved in biological behavior of MDA-MB-231 cell and its mechanisms deserves further research.