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中华乳腺病杂志(电子版)

中华乳腺病杂志(电子版) ›› 2017, Vol. 11 ›› Issue (05) : 272 -276. doi: 10.3877/cma.j.issn.1674-0807.2017.05.004

论著

前列腺干细胞抗原在不同性质乳腺组织中的表达及其意义
郝金燕1, 刘莉萍1,†(), 宫丽平2, 苏雅洁3, 潘慧1, 王晨1, 赵建民1, 刘文悦1   
  1. 1.101300 北京市顺义区医院病理科
    2.100069 北京,首都医科大学病理教研室
    3.101300 北京市顺义区妇幼保健院病理科
  • 收稿日期:2016-11-07 出版日期:2017-10-01
  • 通信作者: 刘莉萍

Expression of prostate stem cell antigen in different breast tissues and its clinical significance

Jinyan Hao1, Liping Liu1,†(), Liping Gong2, Yajie Su3, Hui Pan1, Chen Wang1, Jianmin Zhao1, Wenyue Liu1   

  1. 1.Department of Pathology,Shunyi District Hospital,Beijing 101300,China
    2.Department of Pathology,Capital Medical University, Beijing 100069, China
    3.Department of Pathology, Women and Children's Hospital of Shunyi District, Beijing 101300, China
  • Received:2016-11-07 Published:2017-10-01
  • Corresponding author: Liping Liu
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郝金燕, 刘莉萍, 宫丽平, 苏雅洁, 潘慧, 王晨, 赵建民, 刘文悦. 前列腺干细胞抗原在不同性质乳腺组织中的表达及其意义[J/OL]. 中华乳腺病杂志(电子版), 2017, 11(05): 272-276.

Jinyan Hao, Liping Liu, Liping Gong, Yajie Su, Hui Pan, Chen Wang, Jianmin Zhao, Wenyue Liu. Expression of prostate stem cell antigen in different breast tissues and its clinical significance[J/OL]. Chinese Journal of Breast Disease(Electronic Edition), 2017, 11(05): 272-276.

目的

探讨前列腺干细胞抗原(PSCA)在不同性质乳腺组织中的表达和临床意义。

方法

回顾性分析2000 年1 月至2014 年12 月北京市顺义区医院病理科诊断的、乳腺外科手术切除的、术前未经任何治疗的普通型导管增生(UDH)、非典型导管增生(ADH)、DCIS 和非特殊型浸润性癌(IC-NST)患者共175 例临床资料,其中有38 例因组织块过小或脱落未能获得准确数据而被剔除,故实际纳入分析的样本量为137 例,包括20 例UDH、20 例ADH、30 例DCIS 和67 例IC-NST。 同时随机选取20 例IC-NST 癌旁正常乳腺组织作为对照。 应用免疫组织化学染色法(LSAB 法)检测标本中PSCA 蛋白的表达情况,分析不同性质乳腺组织中PSCA 蛋白表达的差异,以及IC-NST 中PSCA 蛋白表达与临床病理学特征的相关性。 组间PSCA 蛋白表达水平的比较采用χ2 检验,IC-NST 中PSCA 蛋白表达与临床病理学特征的关系采用Spearman 秩相关分析。

结果

PSCA 蛋白在正常乳腺组织中不表达,在UDH、ADH、DCIS 和IC-NST 中高表达率分别为10.0% (2/20)、20.0% (4/20)、56.7% (17/30)和52.2%(35/67),其表达水平的差异具有统计学意义(χ2=32.153,P<0.001)。 多组间两两比较结果显示,DCIS组和IC-NST 组PSCA 蛋白表达均高于正常乳腺组织(χ2=17.172、17.480,P 均<0.005)和UDH 组(χ2=11.092、11.243,P 均<0.005)。 Spearman 秩相关分析显示,IC-NST 组PSCA 蛋白表达与淋巴结转移呈正相关(r=0.326, P= 0.007)。

结论

PSCA 蛋白可能参与了乳腺癌的发生、发展进程,可能是一个预测导管内增生性病变进展成乳腺癌的新指标。

关键词: 乳腺, 人, 淋巴转移, 膜糖蛋白类, 肿瘤蛋白质类, 免疫组织化学, 前列腺干细胞抗原

Objective

To study the expression of prostate stem cell antigen (PSCA) in different breast tissues and its clinical significance.

Methods

We retrospectively analyzed the clinical data of 175 patients who were diagnosed in Department of Pathology, received no treatment before operation and underwent surgical resection in Department of Breast Surgery, Shunyi District Hospital from January 2000 to December 2014,including the patients with usual ductal hyperplasia (UDH), atypical ductal hyperplasia (ADH), ductal carcinoma in situ (DCIS) and invasive carcinoma of no specific type (IC-NST). Thirty-eight cases were excluded from the study for small or abscised samples. Totally 137 cases were enrolled, including 20 cases of UDH,20 ADH,30 DCIS and 67 IC-NST. The adjacent normal tissues from 20 cases of IC-NST served as control. The expression of PSCA in specimens was detected by immunohistochemical staining (LSAB method).The relationship between PSCA expression in different breast tissues and clinicopathological characteristics of the patients was analyzed. PSCA expressions between groups were compared using χ2 test. The relationship between PSCA expression and clinical characteristics of IC-NST patients was analyzed using Spearman rank correlation analysis.

Results

PSCA was not expressed in normal breast tissue. The high expression rates of PSCA in UDH,ADH,DCIS and IC-NST were 10.0% (2/20),20.0%(4/20),56.7%(17/30) and 52.2%(35/67) respectively, indicating a significant difference (χ2=32.153, P<0.001). The expression of PSCA in DCIS or IC-NST was significantly higher than that in normal tissue (χ2=17.172,17.480; both P<0.005)and the expression of PSCA in DCIS or IC-NST was significantly higher than that in UDH (χ2 =11.092,11.243, both P<0.005). Spearman correlation analysis showed that the expression of PSCA was positively correlated with lymph node metastasis in IC-NST(r=0.326, P=0.007).

Conclusion

PSCA may play a role in the occurrence and development of breast cancer and can serve as a new predictor of ductal proliferative lesions being developed into breast cancer.

Key words: Mammary glands, human, Lymphatic metastasis, Membrane glycoproteins, Neoplasm proteins, Immunohistochemistry, Prostate stem cell antigen
表1 不同性质乳腺组织中PSCA 蛋白表达情况
组织类型 例数 PSCA[例(%)]
低表达/阴性 高表达
正常乳腺组织 20 20(100) 0(0)a
UDH 20 18(90.0) 2(10.0)b
ADH 20 16(80.0) 4(20.0)
DCIS 30 13(43.3) 17(56.7)
IC-NST 67 32(47.8) 35(52.2)
χ 2值 32.153
P值 ＜0.001
图1 前列腺干细胞抗原(PSCA)在不同性质乳腺组织中的表达情况
表2 67 例乳腺IC-NST 中PSCA 蛋白表达与临床病理学特征的关系
临床病理学特征 例数 PSCA(例) r值 P值
阴性/低表达 高表达
肿瘤大小
＜2cm 32 15 17 0.028 0.822
2~5cm 32 17 15
＞5cm 3 0 3
临床分期
Ⅰ 19 11 8 0.194 0.115
Ⅱ 39 19 20
Ⅲ 9 2 7
淋巴结转移
阴性 39 24 15 0.326 0.007
阳性 28 8 20
ER
阴性 30 18 12 0.221 0.073
阳性 37 14 23
PR
阴性 33 18 15 0.134 0.280
阳性 34 14 20
HER-2
0/1+ 49 21 28 -0.162 0.190
2+/3+ 18 11 7
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